Key features and details
- Rabbit polyclonal to H-ERA
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-H-ERA antibody
See all H-ERA primary antibodies
DescriptionRabbit polyclonal to H-ERA
Tested applicationsSuitable for: WBmore details
Unsuitable for: IP
Species reactivityReacts with: Human
Synthetic peptide within Human H-ERA aa 75-125. The exact sequence is proprietary.
Database link: O75616
- WB: HeLa, HEK-293T and Jurkat whole cell lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntibody was affinity purified using an epitope specific to H-ERA immobilized on solid support.
Our Abpromise guarantee covers the use of ab241587 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 48 kDa.|
FunctionProbable GTPase that plays a role in the mitochondrial ribosomal small subunit assembly. Specifically binds the 12S mitochondrial rRNA (12S mt-rRNA) to a 33 nucleotide section delineating the 3' terminal stem-loop region. May act as a chaperone that protects the 12S mt-rRNA on the 28S mitoribosomal subunit during ribosomal small subunit assembly.
Sequence similaritiesBelongs to the Era/MnmE GTP-binding protein family. Era subfamily.
Contains 1 G (guanine nucleotide-binding) domain.
Contains 1 KH type-2 domain.
Cellular localizationMitochondrion matrix. Mitochondrion inner membrane. Localizes on the matrix side on the mitochondrial inner membrane.
- Information by UniProt
- CEGA antibody
- Conserved ERA like GTPase antibody
- Conserved ERA-like GTPase antibody
All lanes : Anti-H-ERA antibody (ab241587) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 48 kDa
Exposure time: 3 minutes
Lysates prepared in NETN buffer.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab241587 has not yet been referenced specifically in any publications.