Overview

  • Product name

  • Description

    Rabbit polyclonal to H-ERA
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human H-ERA aa 75-125. The exact sequence is proprietary.
    Database link: O75616

  • Positive control

    • WB: HeLa, HEK-293T and Jurkat whole cell lysates.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Antibody was affinity purified using an epitope specific to H-ERA immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab241587 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 48 kDa.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      Probable GTPase that plays a role in the mitochondrial ribosomal small subunit assembly. Specifically binds the 12S mitochondrial rRNA (12S mt-rRNA) to a 33 nucleotide section delineating the 3' terminal stem-loop region. May act as a chaperone that protects the 12S mt-rRNA on the 28S mitoribosomal subunit during ribosomal small subunit assembly.
    • Sequence similarities

      Belongs to the Era/MnmE GTP-binding protein family. Era subfamily.
      Contains 1 G (guanine nucleotide-binding) domain.
      Contains 1 KH type-2 domain.
    • Cellular localization

      Mitochondrion matrix. Mitochondrion inner membrane. Localizes on the matrix side on the mitochondrial inner membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • CEGA antibody
      • Conserved ERA like GTPase antibody
      • Conserved ERA-like GTPase antibody
      • ERA antibody
      • Era (E. coli G protein homolog) like 1 antibody
      • Era G protein like 1 (E. coli) antibody
      • Era G protein like 1 antibody
      • ERA W antibody
      • ERA-like protein 1 antibody
      • ERA-W antibody
      • Eral1 antibody
      • ERAL1_HUMAN antibody
      • ERAL1A antibody
      • ERAW antibody
      • GTP binding protein era homolog antibody
      • GTPase Era antibody
      • GTPase Era, mitochondrial antibody
      • GTPase human homolog of E. coli essential cell cycle protein Era antibody
      • H-ERA antibody
      • HERA A antibody
      • hERA antibody
      • HERA B antibody
      • HERAA antibody
      • HERAB antibody
      • mitochondrial antibody
      see all

    Images

    • All lanes : Anti-H-ERA antibody (ab241587) at 0.1 µg/ml

      Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
      Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
      Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate

      Lysates/proteins at 50 µg per lane.

      Developed using the ECL technique.

      Predicted band size: 48 kDa


      Exposure time: 3 minutes


      Lysates prepared in NETN buffer.

    References

    ab241587 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab241587.
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