Product nameAnti-H-ERA antibody
See all H-ERA primary antibodies
DescriptionRabbit polyclonal to H-ERA
Tested applicationsSuitable for: WBmore details
Unsuitable for: IP
Species reactivityReacts with: Human
Synthetic peptide within Human H-ERA aa 75-125. The exact sequence is proprietary.
Database link: O75616
- WB: HeLa, HEK-293T and Jurkat whole cell lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntibody was affinity purified using an epitope specific to H-ERA immobilized on solid support.
Our Abpromise guarantee covers the use of ab241587 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 48 kDa.|
FunctionProbable GTPase that plays a role in the mitochondrial ribosomal small subunit assembly. Specifically binds the 12S mitochondrial rRNA (12S mt-rRNA) to a 33 nucleotide section delineating the 3' terminal stem-loop region. May act as a chaperone that protects the 12S mt-rRNA on the 28S mitoribosomal subunit during ribosomal small subunit assembly.
Sequence similaritiesBelongs to the Era/MnmE GTP-binding protein family. Era subfamily.
Contains 1 G (guanine nucleotide-binding) domain.
Contains 1 KH type-2 domain.
Cellular localizationMitochondrion matrix. Mitochondrion inner membrane. Localizes on the matrix side on the mitochondrial inner membrane.
- Information by UniProt
- CEGA antibody
- Conserved ERA like GTPase antibody
- Conserved ERA-like GTPase antibody
All lanes : Anti-H-ERA antibody (ab241587) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 48 kDa
Exposure time: 3 minutes
Lysates prepared in NETN buffer.
ab241587 has not yet been referenced specifically in any publications.