Anti-H-ERG antibody (ab196301)
Key features and details
- Rabbit polyclonal to H-ERG
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-H-ERG antibody
See all H-ERG primary antibodies -
Description
Rabbit polyclonal to H-ERG -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human H-ERG conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Database link: Q12809 -
Positive control
- HeLa whole cell lysate (ab150035) and Human heart tissue
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: 1% BSA -
Concentration information loading...
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Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab196301 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/250 - 1/500. Predicted molecular weight: 127 kDa.
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IHC-P |
1/50 - 1/500.
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Notes |
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WB
1/250 - 1/500. Predicted molecular weight: 127 kDa. |
IHC-P
1/50 - 1/500. |
Target
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Function
Pore-forming (alpha) subunit of voltage-gated inwardly rectifying potassium channel. Channel properties are modulated by cAMP and subunit assembly. Mediates the rapidly activating component of the delayed rectifying potassium current in heart (IKr). Isoform 3 has no channel activity by itself, but modulates channel characteristics when associated with isoform 1. -
Tissue specificity
Highly expressed in heart and brain. -
Involvement in disease
Defects in KCNH2 are the cause of long QT syndrome type 2 (LQT2) [MIM:613688]. Long QT syndromes are heart disorders characterized by a prolonged QT interval on the ECG and polymorphic ventricular arrhythmias. They cause syncope and sudden death in response to exercise or emotional stress. Deafness is often associated with LQT2.
Defects in KCNH2 are the cause of short QT syndrome type 1 (SQT1) [MIM:609620]. Short QT syndromes are heart disorders characterized by idiopathic persistently and uniformly short QT interval on ECG in the absence of structural heart disease in affected individuals. They cause syncope and sudden death. -
Sequence similarities
Belongs to the potassium channel family. H (Eag) (TC 1.A.1.20) subfamily. Kv11.1/KCNH2 sub-subfamily.
Contains 1 cyclic nucleotide-binding domain.
Contains 1 PAC (PAS-associated C-terminal) domain.
Contains 1 PAS (PER-ARNT-SIM) domain. -
Domain
The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position. -
Post-translational
modificationsPhosphorylated on serine and threonine residues. Phosphorylation by PKA inhibits ion conduction. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 3757 Human
- Omim: 152427 Human
- SwissProt: Q12809 Human
- Unigene: 647099 Human
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Alternative names
- eag homolog antibody
- Eag-related protein 1 antibody
- ERG antibody
see all
Images
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab196301 has been referenced in 4 publications.
- Luo X et al. Effects of Vitamin D Deficiency on the Function of the Cardiac Autonomic Nervous System in Rats. Cardiovasc Ther 2022:4366948 (2022). PubMed: 35387268
- Xu T et al. Anticancer effect of miR-96 inhibitor in bladder cancer cell lines. Oncol Lett 15:3814-3819 (2018). PubMed: 29467898
- Jiang Q et al. Identification of small-molecule ion channel modulators in C. elegans channelopathy models. Nat Commun 9:3941 (2018). PubMed: 30258187
- Zeng W et al. Silencing of hERG1 Gene Inhibits Proliferation and Invasion, and Induces Apoptosis in Human Osteosarcoma Cells by Targeting the NF-?B Pathway. J Cancer 7:746-57 (2016). IHC-P, WB ; Human . PubMed: 27076857