Anti-H-FABP antibody [67D3] (ab16916)
Key features and details
- Mouse monoclonal [67D3] to H-FABP
- Suitable for: IHC-P, IHC-Fr
- Reacts with: Human
- Isotype: IgG1
Overview
-
Product name
Anti-H-FABP antibody [67D3]
See all H-FABP primary antibodies -
Description
Mouse monoclonal [67D3] to H-FABP -
Host species
Mouse -
Specificity
Ab16916 binds to human H-FABP. It shows no binding to human intestinal or liver FABP and slightly cross reacts with B-FABP.
-
Tested applications
Suitable for: IHC-P, IHC-Frmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Full length native protein (purified) corresponding to Human H-FABP.
-
Positive control
- Heart cells, recombinant human H-FABP This antibody gave a positive result in IHC in the following FFPE tissue: Human normal heart muscle.
-
General notes
This antibody can be used to detect ischemia areas of human heart. It is also useful as marker for brain damage.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituents: PBS, 0.1% BSA -
Concentration information loading...
-
Purity
Protein G purified -
Purification notes
Provided as a 0.2µm filtered antibody solution. -
Primary antibody notes
This antibody can be used to detect ischemia areas of human heart. It is also useful as marker for brain damage. -
Clonality
Monoclonal -
Clone number
67D3 -
Isotype
IgG1 -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab16916 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P | (1) |
Use at an assay dependent concentration.
|
IHC-Fr |
Use at an assay dependent concentration.
|
Notes |
---|
IHC-P
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
Target
-
Function
FABP are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters. -
Sequence similarities
Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family. -
Domain
Forms a beta-barrel structure that accommodates the hydrophobic ligand in its interior. -
Cellular localization
Cytoplasm. - Information by UniProt
-
Database links
- Entrez Gene: 2170 Human
- Entrez Gene: 14077 Mouse
- Entrez Gene: 79131 Rat
- Omim: 134651 Human
- SwissProt: P05413 Human
- SwissProt: P11404 Mouse
- SwissProt: P07483 Rat
- Unigene: 657242 Human
see all -
Alternative names
- 422 protein antibody
- Cardiac FABP antibody
- Cardiac Fatty Acid Binding Protein antibody
see all
Images
-
Immunohistochemical staining of H-FABP in human skeletal muscle tissue.
-
IHC image of H-FABP staining in Human normal heart muscle formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab16916, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Datasheets and documents
-
Datasheet download
References (6)
ab16916 has been referenced in 6 publications.
- Belcastro L et al. Decreased Fatty Acid Transporter FABP1 and Increased Isoprostanes and Neuroprostanes in the Human Term Placenta: Implications for Inflammation and Birth Weight in Maternal Pre-Gestational Obesity. Nutrients 13:N/A (2021). PubMed: 34444927
- Khan D et al. SIRT6 transcriptionally regulates fatty acid transport by suppressing PPARγ. Cell Rep 35:109190 (2021). PubMed: 34077730
- Jones HB et al. Myocardial Steatosis and Necrosis in Atria and Ventricles of Rats Given Pyruvate Dehydrogenase Kinase Inhibitors. Toxicol Pathol N/A:N/A (2014). PubMed: 24742628
- Chen HM et al. Heart-type Fatty Acid binding protein is associated with proteinuria in obesity. PLoS One 7:e45691 (2012). IHC-P, IHC-Fr ; Mouse . PubMed: 23029183
- Heather LC et al. Adenosine monophosphate-activated protein kinase activation, substrate transporter translocation, and metabolism in the contracting hyperthyroid rat heart. Endocrinology 151:422-31 (2010). WB ; Rat . PubMed: 19940039
- Nevo J et al. Mammary-derived growth inhibitor alters traffic of EGFR and induces a novel form of cetuximab resistance. Clin Cancer Res 15:6570-81 (2009). IHC-P ; Human . PubMed: 19825952