Overview

  • Product name
    Anti-H-FABP antibody [67D3]
    See all H-FABP primary antibodies
  • Description
    Mouse monoclonal [67D3] to H-FABP
  • Host species
    Mouse
  • Specificity
    Ab16916 binds to human cardiac FABP. It shows no binding to human intestinal or liver FABP and slightly cross reacts with B-FABP.
  • Tested applications
    Suitable for: IHC-Fr, IP, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Full length native protein (purified) corresponding to Human H-FABP.

  • Positive control
    • Heart cells, recombinant human H-FABP This antibody gave a positive result in IHC in the following FFPE tissue: Human normal heart muscle.
  • General notes


    This antibody can be used to detect ischemia areas of human heart. It is also useful as marker for brain damage.

Properties

Applications

Our Abpromise guarantee covers the use of ab16916 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration. PubMed: 23029183
IP Use at an assay dependent concentration.
IHC-P 1/10. PubMed: 19825952
WB Use at an assay dependent concentration. Use under non reducing condition. Predicted molecular weight: 15 kDa.

Target

  • Function
    FABP are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters.
  • Sequence similarities
    Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.
  • Domain
    Forms a beta-barrel structure that accommodates the hydrophobic ligand in its interior.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • 422 protein antibody
    • Cardiac FABP antibody
    • Cardiac Fatty Acid Binding Protein antibody
    • FABP 11 antibody
    • FABP 3 antibody
    • FABP11 antibody
    • FABP3 antibody
    • FABPH_HUMAN antibody
    • Fatty acid binding protein 11 antibody
    • Fatty acid binding protein 3 antibody
    • Fatty acid binding protein 3 muscle and heart antibody
    • Fatty acid binding protein 3 muscle and heart mammary derived growth inhibitor antibody
    • Fatty acid binding protein 3 muscle antibody
    • Fatty acid binding protein 3, muscle and heart (mammary derived growth inhibitor) antibody
    • Fatty acid binding protein 3, muscle antibody
    • Fatty acid binding protein heart antibody
    • Fatty acid binding protein, heart antibody
    • Fatty acid binding protein, muscle and heart antibody
    • Fatty acid binding protein, skeletal muscle antibody
    • Fatty acid-binding protein 3 antibody
    • Fatty acid-binding protein antibody
    • H FABP antibody
    • H-FABP antibody
    • heart antibody
    • Heart type fatty acid binding protein antibody
    • Heart-type fatty acid-binding protein antibody
    • M FABP antibody
    • M-FABP antibody
    • Mammary derived growth inhibitor antibody
    • Mammary-derived growth inhibitor antibody
    • MDGI antibody
    • Muscle fatty acid binding protein antibody
    • Muscle fatty acid-binding protein antibody
    • Mylein protein P2 homolog antibody
    • O FABP antibody
    • OTTHUMP00000003898 antibody
    • P2 adopocyte protein antibody
    see all

Images

  • Immunohistochemical staining of H-FABP in human skeletal muscle tissue.

  • IHC image of H-FABP staining in Human normal heart muscle formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab16916, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References

This product has been referenced in:
  • Jones HB  et al. Myocardial Steatosis and Necrosis in Atria and Ventricles of Rats Given Pyruvate Dehydrogenase Kinase Inhibitors. Toxicol Pathol N/A:N/A (2014). Read more (PubMed: 24742628) »
  • Chen HM  et al. Heart-type Fatty Acid binding protein is associated with proteinuria in obesity. PLoS One 7:e45691 (2012). IHC-P, IHC-Fr ; Mouse . Read more (PubMed: 23029183) »
See all 4 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Heart)
Antigen retrieval step
None
Permeabilization
No
Specification
Heart
Blocking step
Serum free as blocking agent for 15 hour(s) and 0 minute(s) · Concentration: 0µg/mL · Temperature: 24°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Feb 26 2009

Question
Answer

Thank you for your recent telephone enquiry. I have received confirmation that this antibody is cross reactive with rat and mouse H-FABP (Isoform FABP3). I hope this information is helpful. Should you have any further questions, please do not hesitate to contact us again.

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Answer

Thank you for your enquiry regarding ab16916. The monoclonal antibody binds to human heart FABP. It shows no binding to human intestinal or liver FABP. Western blot analysis showed that the antibody slightly cross reacts with B-FABP. For immunohistology and Western blotting dilutions to be used depending on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. The dilution 1:10 is without amplification with ABC so you can maybe dilute more by using ABC. The antibody is tested for Western blotting under non-reduced conditions. It is possible that denaturing conditions give difficulties however this is never been tested. We are very sorry but ab18062 is no longer included in our product range, I apologise for the inconvenience, We hope this information will be helpful to you. If you have further questions please do not hesitate to contact us.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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