Key features and details
- Rabbit polyclonal to H-FABP
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-H-FABP antibody
See all H-FABP primary antibodies
DescriptionRabbit polyclonal to H-FABP
ab45966 reacts with H-FABP, but does not react with liver or intestinal forms of this protein.
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Cow, Pig
Synthetic peptide corresponding to Human H-FABP aa 100 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available as
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab45966 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 15 kDa (predicted molecular weight: 15 kDa).|
FunctionFABP are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters.
Sequence similaritiesBelongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.
DomainForms a beta-barrel structure that accommodates the hydrophobic ligand in its interior.
- Information by UniProt
- 422 protein antibody
- Cardiac FABP antibody
- Cardiac Fatty Acid Binding Protein antibody
All lanes : Anti-H-FABP antibody (ab45966) at 1 µg/ml
Lane 1 : Human liver tissue lysate - total protein (ab29889)
Lane 2 : Human heart tissue lysate - total protein (ab29431)
Lane 3 : Human skeletal muscle tissue lysate - total protein (ab29330)
Lane 4 : Human small intestine tissue lysate - total protein (ab29276)
Lysates/proteins at 10 µg per lane.
All lanes : Rabbit IgG secondary antibody (ab28446) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 15 kDa
Observed band size: 15 kDa
Ab45966 does not cross-react with small intestine or liver FABP.
Ab45966 recognizes the full length tagged recombinant protein (ab109160) which has an expected molecular weight of 16 kDa.
ab45966 has been referenced in 10 publications.
- Chassen SS et al. Placental fatty acid transport across late gestation in a baboon model of intrauterine growth restriction. J Physiol 598:2469-2489 (2020). PubMed: 32338384
- Baati N et al. New evidence of exercise training benefits in myostatin-deficient mice: Effect on lipidomic abnormalities. Biochem Biophys Res Commun 516:89-95 (2019). PubMed: 31200956
- Shrestha N et al. Linoleic Acid Increases Prostaglandin E2 Release and Reduces Mitochondrial Respiration and Cell Viability in Human Trophoblast-Like Cells. Cell Physiol Biochem 52:94-108 (2019). PubMed: 30790507
- Šrámek J et al. Hypoxia Modulates Effects of Fatty Acids on NES2Y Human Pancreatic ß-cells. Int J Mol Sci 20:N/A (2019). PubMed: 31336948
- Baati N et al. Myostatin deficiency is associated with lipidomic abnormalities in skeletal muscles. Biochim Biophys Acta 1862:1044-1055 (2017). WB ; Mouse . PubMed: 28676454
- Del Collado M et al. Fatty Acid Binding Protein 3 And Transzonal Projections Are Involved In Lipid Accumulation During In Vitro Maturation Of Bovine Oocytes. Sci Rep 7:2645 (2017). WB . PubMed: 28572619
- Li S et al. Betaine affects muscle lipid metabolism via regulating the fatty acid uptake and oxidation in finishing pig. J Anim Sci Biotechnol 8:72 (2017). WB ; Pig . PubMed: 28883917
- Banu SK et al. Editor's Highlight: Exposure to CrVI during Early Pregnancy Increases Oxidative Stress and Disrupts the Expression of Antioxidant Proteins in Placental Compartments. Toxicol Sci 155:497-511 (2017). PubMed: 28077780
- Tsukahara R et al. Heart-type fatty-acid-binding protein (FABP3) is a lysophosphatidic acid-binding protein in human coronary artery endothelial cells. FEBS Open Bio 4:947-51 (2014). PubMed: 25426414
- Antohe F et al. Heart microvessels and aortic endothelial cells express the 15 kDa heart-type fatty acid-binding proteins. Eur J Cell Biol 76:102-9 (1998). IHC-P ; Rat . PubMed: 9696349