Product nameAnti-H-FABP antibody
See all H-FABP primary antibodies
DescriptionRabbit polyclonal to H-FABP
Specificityab45966 reacts with cardiac FABP, but does not react with liver or intestinal forms of this protein.
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Cow, Pig
Synthetic peptide corresponding to Human H-FABP aa 100 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available as
- Recombinant Human H-FABP protein (ab84335) can be used as a positive control in WB. ab45966 gives a positive result in the following tissue lysates: Heart (Human) Tissue Lysate, Skeletal Muscle (Human) Tissue Lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab45966 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 15 kDa (predicted molecular weight: 15 kDa).|
|IHC-P||1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
FunctionFABP are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters.
Sequence similaritiesBelongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.
DomainForms a beta-barrel structure that accommodates the hydrophobic ligand in its interior.
- Information by UniProt
- 422 protein antibody
- Cardiac FABP antibody
- Cardiac Fatty Acid Binding Protein antibody
All lanes : Anti-H-FABP antibody (ab45966) at 1 µg/ml
Lane 1 : Human liver tissue lysate - total protein (ab29889)
Lane 2 : Human heart tissue lysate - total protein (ab29431)
Lane 3 : Human skeletal muscle tissue lysate - total protein (ab29330)
Lane 4 : Human small intestine tissue lysate - total protein (ab29276)
Lysates/proteins at 10 µg per lane.
All lanes : Rabbit IgG secondary antibody (ab28446) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 15 kDa
Observed band size: 15 kDa
Ab45966 does not cross-react with small intestine or liver FABP.
ab45966, at a 1/500 dilution, staining H-FABP in mouse heart by Immunohistochemistry, using formalin-fixed paraffin embedded tissue.
Ab45966 recognizes the full length tagged recombinant protein (ab109160) which has an expected molecular weight of 16 kDa.
This product has been referenced in:
- Baati N et al. Myostatin deficiency is associated with lipidomic abnormalities in skeletal muscles. Biochim Biophys Acta 1862:1044-1055 (2017). WB ; Mouse . Read more (PubMed: 28676454) »
- Del Collado M et al. Fatty Acid Binding Protein 3 And Transzonal Projections Are Involved In Lipid Accumulation During In Vitro Maturation Of Bovine Oocytes. Sci Rep 7:2645 (2017). WB . Read more (PubMed: 28572619) »