Recombinant
RabMAb

Recombinant Anti-H-FABP antibody [EPR6126] (ab133585)

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Overview

  • Product name

    Anti-H-FABP antibody [EPR6126]
    See all H-FABP primary antibodies

  • Description

    Rabbit monoclonal [EPR6126] to H-FABP

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WBmore details
    Unsuitable for: ICC or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human H-FABP aa 1-100. The exact sequence is proprietary.

  • Positive control

    • Fetal muscle and fetal heart lysates; Human heart muscle tissue.

  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab133585 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000 - 1/10000. Detects a band of approximately 14 kDa (predicted molecular weight: 15 kDa).
  • Application notes
    Is unsuitable for ICC or IP.

    • Target

    • Function

      FABP are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters.

    • Sequence similarities

      Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.

    • Domain

      Forms a beta-barrel structure that accommodates the hydrophobic ligand in its interior.

    • Cellular localization

      Cytoplasm.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • 422 protein antibody
      • Cardiac FABP antibody
      • Cardiac Fatty Acid Binding Protein antibody
      • FABP 11 antibody
      • FABP 3 antibody
      • FABP11 antibody
      • FABP3 antibody
      • FABPH_HUMAN antibody
      • Fatty acid binding protein 11 antibody
      • Fatty acid binding protein 3 antibody
      • Fatty acid binding protein 3 muscle and heart antibody
      • Fatty acid binding protein 3 muscle and heart mammary derived growth inhibitor antibody
      • Fatty acid binding protein 3 muscle antibody
      • Fatty acid binding protein 3, muscle and heart (mammary derived growth inhibitor) antibody
      • Fatty acid binding protein 3, muscle antibody
      • Fatty acid binding protein heart antibody
      • Fatty acid binding protein, heart antibody
      • Fatty acid binding protein, muscle and heart antibody
      • Fatty acid binding protein, skeletal muscle antibody
      • Fatty acid-binding protein 3 antibody
      • Fatty acid-binding protein antibody
      • H FABP antibody
      • H-FABP antibody
      • heart antibody
      • Heart type fatty acid binding protein antibody
      • Heart-type fatty acid-binding protein antibody
      • M FABP antibody
      • M-FABP antibody
      • Mammary derived growth inhibitor antibody
      • Mammary-derived growth inhibitor antibody
      • MDGI antibody
      • Muscle fatty acid binding protein antibody
      • Muscle fatty acid-binding protein antibody
      • Mylein protein P2 homolog antibody
      • O FABP antibody
      • OTTHUMP00000003898 antibody
      • P2 adopocyte protein antibody
      see all

    Images

    • Western blot - Anti-H-FABP antibody [EPR6126] (ab133585)
      Western blot - Anti-H-FABP antibody [EPR6126] (ab133585)
      All lanes : Anti-H-FABP antibody [EPR6126] (ab133585) at 1/1000 dilution

      Lane 1 : Fetal muscle lysate
      Lane 2 : Fetal heart lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution

      Predicted band size: 15 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-H-FABP antibody [EPR6126] (ab133585)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-H-FABP antibody [EPR6126] (ab133585)

      Immunohistochemical analysis of paraffin embedded Human heart tissue labelling H-FABP with ab133585 at 1/100 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Other - Anti-H-FABP antibody [EPR6126] (ab133585)
      Other - Anti-H-FABP antibody [EPR6126] (ab133585)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    • Che L  et al. Valine increases milk fat synthesis in mammary gland of gilts through stimulating AKT/MTOR/SREBP1 pathway‡. Biol Reprod N/A:N/A (2019). Read more (PubMed: 30985894) »
    See 1 Publication for this product

    Publishing research using ab133585? Please let us know so that we can cite the reference in this datasheet.

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