RecombinantRabMAb

Recombinant Anti-H-FABP antibody [EPR6126] (ab133585)

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Western blot - Anti-H-FABP antibody [EPR6126] (ab133585)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-H-FABP antibody [EPR6126] (ab133585)
  • OI-RD Scanning - Anti-H-FABP antibody [EPR6126] (ab133585)
  • Anti-H-FABP antibody [EPR6126] (ab133585)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6126] to H-FABP
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-H-FABP antibody [EPR6126]
    See all H-FABP primary antibodies

  • Description

    Rabbit monoclonal [EPR6126] to H-FABP

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WBmore details
    Unsuitable for: ICC or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human H-FABP aa 1-100. The exact sequence is proprietary.

  • Positive control

    • Fetal muscle and fetal heart lysates; Human heart muscle tissue.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab133585 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000 - 1/10000. Detects a band of approximately 14 kDa (predicted molecular weight: 15 kDa).
  • Application notes
    Is unsuitable for ICC or IP.

    • Target

    • Function

      FABP are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters.

    • Sequence similarities

      Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.

    • Domain

      Forms a beta-barrel structure that accommodates the hydrophobic ligand in its interior.

    • Cellular localization

      Cytoplasm.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • 422 protein antibody
      • Cardiac FABP antibody
      • Cardiac Fatty Acid Binding Protein antibody
      • FABP 11 antibody
      • FABP 3 antibody
      • FABP11 antibody
      • FABP3 antibody
      • FABPH_HUMAN antibody
      • Fatty acid binding protein 11 antibody
      • Fatty acid binding protein 3 antibody
      • Fatty acid binding protein 3 muscle and heart antibody
      • Fatty acid binding protein 3 muscle and heart mammary derived growth inhibitor antibody
      • Fatty acid binding protein 3 muscle antibody
      • Fatty acid binding protein 3, muscle and heart (mammary derived growth inhibitor) antibody
      • Fatty acid binding protein 3, muscle antibody
      • Fatty acid binding protein heart antibody
      • Fatty acid binding protein, heart antibody
      • Fatty acid binding protein, muscle and heart antibody
      • Fatty acid binding protein, skeletal muscle antibody
      • Fatty acid-binding protein 3 antibody
      • Fatty acid-binding protein antibody
      • H FABP antibody
      • H-FABP antibody
      • heart antibody
      • Heart type fatty acid binding protein antibody
      • Heart-type fatty acid-binding protein antibody
      • M FABP antibody
      • M-FABP antibody
      • Mammary derived growth inhibitor antibody
      • Mammary-derived growth inhibitor antibody
      • MDGI antibody
      • Muscle fatty acid binding protein antibody
      • Muscle fatty acid-binding protein antibody
      • Mylein protein P2 homolog antibody
      • O FABP antibody
      • OTTHUMP00000003898 antibody
      • P2 adopocyte protein antibody
      see all

    Images

    • Western blot - Anti-H-FABP antibody [EPR6126] (ab133585)
      Western blot - Anti-H-FABP antibody [EPR6126] (ab133585)
      All lanes : Anti-H-FABP antibody [EPR6126] (ab133585) at 1/1000 dilution

      Lane 1 : Fetal muscle lysate
      Lane 2 : Fetal heart lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution

      Predicted band size: 15 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-H-FABP antibody [EPR6126] (ab133585)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-H-FABP antibody [EPR6126] (ab133585)

      Immunohistochemical analysis of paraffin embedded Human heart tissue labelling H-FABP with ab133585 at 1/100 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • OI-RD Scanning - Anti-H-FABP antibody [EPR6126] (ab133585)
      OI-RD Scanning - Anti-H-FABP antibody [EPR6126] (ab133585)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD
    • Anti-H-FABP antibody [EPR6126] (ab133585)
      Anti-H-FABP antibody [EPR6126] (ab133585)

    References (2)

    Publishing research using ab133585? Please let us know so that we can cite the reference in this datasheet.

    ab133585 has been referenced in 2 publications.

    • Che L  et al. Valine increases milk fat synthesis in mammary gland of gilts through stimulating AKT/MTOR/SREBP1 pathway‡. Biol Reprod N/A:N/A (2019). PubMed: 30985894
    • Vileigas DF  et al. Landscape of heart proteome changes in a diet-induced obesity model. Sci Rep 9:18050 (2019). PubMed: 31792287

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