Recombinant Anti-H-FABP antibody [EPR6127] (ab124978)
![Western blot - Anti-H-FABP antibody [EPR6127] (ab124978)](https://www.abcam.com/ps/products/124/ab124978/Images/ab124978-139072-anti-h-fabp-antibody-epr6127-western-blot.jpg "Western blot - Anti-H-FABP antibody [EPR6127] (ab124978)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6127] to H-FABP
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-H-FABP antibody [EPR6127]
See all H-FABP primary antibodies -
Description
Rabbit monoclonal [EPR6127] to H-FABP -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC/IF or IP -
Species reactivity
Reacts with: Human -
Immunogen
corresponding to Human H-FABP aa 50-150.
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Positive control
- Fetal muscle and fetal heart lysates and Human heart and skeletal muscle tissues.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR6127 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab124978 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 15 kDa.
|
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| IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Antigen retrieval is recommended. |
| Notes |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 15 kDa. |
|
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval is recommended. |
Target
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Function
FABP are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters. -
Sequence similarities
Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family. -
Domain
Forms a beta-barrel structure that accommodates the hydrophobic ligand in its interior. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 2170 Human
- Omim: 134651 Human
- SwissProt: P05413 Human
- Unigene: 657242 Human
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Alternative names
- 422 protein antibody
- Cardiac FABP antibody
- Cardiac Fatty Acid Binding Protein antibody
see all
Images
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Western blot - Anti-H-FABP antibody [EPR6127] (ab124978)All lanes : Anti-H-FABP antibody [EPR6127] (ab124978) at 1/1000 dilution
Lane 1 : Human fetal muscle lysate
Lane 2 : Human heart lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 15 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-H-FABP antibody [EPR6127] (ab124978)](https://www.abcam.com/ps/products/124/ab124978/Images/ab124978-139071-anti-h-fabp-antibody-epr6127-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-H-FABP antibody [EPR6127] (ab124978)ab124978, at 1/250 to 1/500, staining H-FABP in Human heart tissue by immunohistochemistry [Paraffin Embedded Tissues (IHC-P)].
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-H-FABP antibody [EPR6127] (ab124978)](https://www.abcam.com/ps/products/124/ab124978/Images/ab124978-139070-anti-h-fabp-antibody-epr6127-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-H-FABP antibody [EPR6127] (ab124978)ab124978, at 1/250 to 1/500, staining H-FABP in Human skeletal muscle tissue by immunohistochemistry [Paraffin Embedded Tissues (IHC-P)].
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-H-FABP antibody [EPR6127] (ab124978)](https://www.abcam.com/ps/products/124/ab124978/Images/ab124978-4-benefits-of-recombinant-antibodies.png)
Anti-H-FABP antibody [EPR6127] (ab124978)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab124978 has not yet been referenced specifically in any publications.