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RecombinantRabMAb

Recombinant Anti-H-FABP antibody [EPR6127] (ab124978)

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Western blot - Anti-H-FABP antibody [EPR6127] (ab124978)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-H-FABP antibody [EPR6127] (ab124978)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-H-FABP antibody [EPR6127] (ab124978)
  • Anti-H-FABP antibody [EPR6127] (ab124978)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6127] to H-FABP
  • Suitable for: WB, IHC-P
  • Reacts with: Human

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Overview

  • Product name

    Anti-H-FABP antibody [EPR6127]
    See all H-FABP primary antibodies
  • Description

    Rabbit monoclonal [EPR6127] to H-FABP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    corresponding to Human H-FABP aa 50-150.

  • Positive control

    • Fetal muscle and fetal heart lysates and Human heart and skeletal muscle tissues.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.2
    Preservative: 0.05% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR6127
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Lipids / Lipoproteins
    • Fatty Acids
    • Binding proteins
    • Stem Cells
    • Mesenchymal Stem Cells
    • Myogenesis
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Fatty acids
    • Metabolism
    • Pathways and Processes
    • Redox metabolism
    • Fatty acid oxidation

Associated products

  • Alternative Versions

    • Anti-H-FABP antibody [EPR6127] - BSA and Azide free (ab248085)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human H-FABP protein (ab84335)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab124978 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 15 kDa.
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Antigen retrieval is recommended.

Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 15 kDa.
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Antigen retrieval is recommended.

  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IP.
  • Target

    • Function

      FABP are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters.
    • Sequence similarities

      Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.
    • Domain

      Forms a beta-barrel structure that accommodates the hydrophobic ligand in its interior.
    • Cellular localization

      Cytoplasm.
    • Target information above from: UniProt accession P05413 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 2170 Human
      • Omim: 134651 Human
      • SwissProt: P05413 Human
      • Unigene: 657242 Human
      • Alternative names

        • 422 protein antibody
        • Cardiac FABP antibody
        • Cardiac Fatty Acid Binding Protein antibody
        • FABP 11 antibody
        • FABP 3 antibody
        • FABP11 antibody
        • FABP3 antibody
        • FABPH_HUMAN antibody
        • Fatty acid binding protein 11 antibody
        • Fatty acid binding protein 3 antibody
        • Fatty acid binding protein 3 muscle and heart antibody
        • Fatty acid binding protein 3 muscle and heart mammary derived growth inhibitor antibody
        • Fatty acid binding protein 3 muscle antibody
        • Fatty acid binding protein 3, muscle and heart (mammary derived growth inhibitor) antibody
        • Fatty acid binding protein 3, muscle antibody
        • Fatty acid binding protein heart antibody
        • Fatty acid binding protein, heart antibody
        • Fatty acid binding protein, muscle and heart antibody
        • Fatty acid binding protein, skeletal muscle antibody
        • Fatty acid-binding protein 3 antibody
        • Fatty acid-binding protein antibody
        • H FABP antibody
        • H-FABP antibody
        • heart antibody
        • Heart type fatty acid binding protein antibody
        • Heart-type fatty acid-binding protein antibody
        • M FABP antibody
        • M-FABP antibody
        • Mammary derived growth inhibitor antibody
        • Mammary-derived growth inhibitor antibody
        • MDGI antibody
        • Muscle fatty acid binding protein antibody
        • Muscle fatty acid-binding protein antibody
        • Mylein protein P2 homolog antibody
        • O FABP antibody
        • OTTHUMP00000003898 antibody
        • P2 adopocyte protein antibody
        see all

      Images

      • Western blot - Anti-H-FABP antibody [EPR6127] (ab124978)
        Western blot - Anti-H-FABP antibody [EPR6127] (ab124978)
        All lanes : Anti-H-FABP antibody [EPR6127] (ab124978) at 1/1000 dilution

        Lane 1 : Human fetal muscle lysate
        Lane 2 : Human heart lysate

        Lysates/proteins at 10 µg per lane.

        Predicted band size: 15 kDa

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-H-FABP antibody [EPR6127] (ab124978)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-H-FABP antibody [EPR6127] (ab124978)

        ab124978, at 1/250 to 1/500, staining H-FABP in Human heart tissue by immunohistochemistry [Paraffin Embedded Tissues (IHC-P)].

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-H-FABP antibody [EPR6127] (ab124978)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-H-FABP antibody [EPR6127] (ab124978)

        ab124978, at 1/250 to 1/500, staining H-FABP in Human skeletal muscle tissue by immunohistochemistry [Paraffin Embedded Tissues (IHC-P)].

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Anti-H-FABP antibody [EPR6127] (ab124978)
        Anti-H-FABP antibody [EPR6127] (ab124978)

      Protocols

      • Western blot protocols
      • Immunohistochemistry protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (0)

      Publishing research using ab124978? Please let us know so that we can cite the reference in this datasheet.

      ab124978 has not yet been referenced specifically in any publications.

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