Overview

  • Product name

  • Description

    Rabbit polyclonal to H-protein
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Immunomicroscopymore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to Human H-protein aa 107-156 (N terminal).
    Sequence:

    LELCREGASEWVPVSARPMMVTQQTVRNLALGDKFLLRVSAVSSAGAGPP


    Database link: NP_004988

  • Positive control

    • HeLa cell lysate.
  • General notes

    Previously labelled as MYBPH.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab98187 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 52 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Immunomicroscopy Use at an assay dependent concentration. PubMed: 24184715

Target

  • Function

    Binds to myosin; probably involved in interaction with thick myofilaments in the A-band.
  • Tissue specificity

    Skeletal muscle.
  • Sequence similarities

    Belongs to the immunoglobulin superfamily. MyBP family.
    Contains 2 fibronectin type-III domains.
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
  • Information by UniProt
  • Database links

  • Alternative names

    • bHLHe38 antibody
    • H protein antibody
    • H-protein antibody
    • l-myc protein antibody
    • l-myc-1 proto-oncogene antibody
    • LMYC antibody
    • MyBP H antibody
    • MyBP-H antibody
    • Mybph antibody
    • MYBPH_HUMAN antibody
    • myc-related gene from lung cancer antibody
    • MYCL antibody
    • Myosin binding protein H antibody
    • Myosin binding protein, 86-KD antibody
    • Myosin-binding protein H antibody
    • Oncogene lmyc antibody
    • V-myc avian myelocytomatosis viral oncogene homolog 1, lung carcinoma derived antibody
    see all

Images

  • Anti-H-protein antibody (ab98187) at 1 µg/ml (n 5% skim milk / PBS buffer) + HeLa Cell Lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 52 kDa



    12% SDS PAGE

References

This product has been referenced in:

  • Conti A  et al. Increased expression of Myosin binding protein H in the skeletal muscle of amyotrophic lateral sclerosis patients. Biochim Biophys Acta 1842:99-106 (2014). IP ; Human . Read more (PubMed: 24184715) »
See 1 Publication for this product

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