Key features and details
- Mouse monoclonal [OTI3G1] to H-protein
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG2a
Product nameAnti-H-protein antibody [OTI3G1]
DescriptionMouse monoclonal [OTI3G1] to H-protein
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Recombinant full length protein corresponding to Human H-protein aa 1-477. H-protein (NP_004988) produced in HEK-293T cell.
MMEKNTSEGPACSPEETASESAKVPTAEPPGEVAVSESTREEQVPKPQAP APQAPTASTATKPAPPSEDVPSAPLLLTLDDVSSSSVTVSWEPPERLGRL GLQGYVLELCREGASEWVPVSARPMMVTQQTVRNLALGDKFLLRVSAVSS AGAGPPAMLDQPIHIRENIEAPKIRVPRHLRQTYIRQVGETVNLQIPFQG KPKPQATWTHNGHALDSQRVSMRTGDQDSILFIRSAQRSDSGRYELTVRV EDLEAKAVIDILVIEKPGPPSSIRLLDVWGCNAALQWTPPQDTGNTELLG YMVQKADKKTGQWFTVLERYHPTTCTISDLIIGNSYSFRVFSENLCGLST SATVTKELAHIQKADIAAKPKGFIERDFSEAPSFTQPLADHTSTPGYSTQ LFCSVRASPKPKIIWMKNKMEIQGNPKYRALSEQGVCTLEIRKPSPFDSG VYTCKAINVLGEASVDCRLEVKASAAH
Database link: Q13203
- Human colon adenocarcinoma tissue, Human lung carcinoma tissue and Human pancreas tissue. HeLa cells.
The clone number has been updated from 3G1 to OTI3G1, both clone numbers name the same clone.
Previously labelled as MYBPH.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 1% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab156763 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/4000. Predicted molecular weight: 52 kDa.|
FunctionBinds to myosin; probably involved in interaction with thick myofilaments in the A-band.
Tissue specificitySkeletal muscle.
Sequence similaritiesBelongs to the immunoglobulin superfamily. MyBP family.
Contains 2 fibronectin type-III domains.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
- Information by UniProt
- bHLHe38 antibody
- H protein antibody
- H-protein antibody
All lanes : Anti-H-protein antibody [OTI3G1] (ab156763) at 1/4000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with pCMV6-ENTRY control
Lane 2 : HEK-293T cells transfected with pCMV6-ENTRY H-protein
Lysates/proteins at 5 µg per lane.
Predicted band size: 52 kDa
Immunofluorescence analysis of COS7 cells transiently transfected by pCMV6-ENTRY H-protein labeling H-protein with ab156763 at 1/100.
Paraffin embedded Human colon adenocarcinoma tissue labeling H-protein with ab156763 at 1/150 in immunohistochemical analysis.
Paraffin embedded Human lung carcinoma tissue labeling H-protein with ab156763 at 1/150 in immunohistochemical analysis.
Paraffin embedded Human pancreas tissue labeling H-protein with ab156763 at 1/150 in immunohistochemical analysis.
ab156763 has not yet been referenced specifically in any publications.