Key features and details
- Rabbit polyclonal to H1N1 Influenza A virus Nucleocapsid protein
- Suitable for: WB, ELISA, ICC/IF
- Reacts with: Influenza A
- Isotype: IgG
Product nameAnti-H1N1 Influenza A virus Nucleocapsid protein antibody
See all H1N1 Influenza A virus Nucleocapsid protein primary antibodies
DescriptionRabbit polyclonal to H1N1 Influenza A virus Nucleocapsid protein
Tested applicationsSuitable for: WB, ELISA, ICC/IFmore details
Species reactivityReacts with: Influenza A
Synthetic peptide derived from internal part of H1N1 Influenza A virus Nucleocapsid protein.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferConstituent: Whole serum
Concentration information loading...
Our Abpromise guarantee covers the use of ab104870 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 56 kDa.
Dilute 1:200 - 1:2000
|ELISA||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.|
RelevanceEncapsidates the negative strand viral RNA, protecting it from nucleases. The encapsidated genomic RNA is termed the ribonucleoprotein (RNP) and serves as template for transcription and replication. The RNP needs to be localized in the nucleus to start an infectious cycle, but is too large to diffuse through the nuclear pore complex. NP comprises at least 2 nuclear localization signals and is responsible of the active RNP import into the nucleus through the cellular importin alpha/beta pathway. Later in the infection, nucleus export of RNP are mediated through viral proteins NEP interacting with M1 which binds nucleoproteins. It is possible that the nucleoprotein binds directly exportin-1 (XPO1) and plays an active role in RNP nuclear export. M1 interaction with RNP seems to hide nucleoprotein's nuclear localization signals. Soon after a virion infects a new cell, M1 dissociates from the RNP under acidification of the virion driven by M2 protein. Dissociation of M1 from RNP unmask nucleoprotein's nuclear localization signals, targeting the RNP to the nucleus.
Cellular localizationVirion. Host nucleus
- SwissProt: P03466 Influenza A
- NP antibody
- Nucleocapsid protein antibody
- Nucleoprotein antibody
- Protein N antibody
ab104870 has been referenced in 2 publications.
- Ren L et al. Influenza A virus (H1N1) triggers a hypoxic response by stabilizing hypoxia-inducible factor-1a via inhibition of proteasome. Virology 530:51-58 (2019). PubMed: 30780125
- Švancarová P & Betáková T Conserved methionine 165 of matrix protein contributes to the nuclear import and is essential for influenza A virus replication. Virol J 15:187 (2018). PubMed: 30509291