Anti-H1N1 Influenza A virus Nucleocapsid protein antibody (ab104870)
Key features and details
- Rabbit polyclonal to H1N1 Influenza A virus Nucleocapsid protein
- Suitable for: WB, ELISA, ICC/IF
- Reacts with: Influenza A
- Isotype: IgG
Overview
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Product name
Anti-H1N1 Influenza A virus Nucleocapsid protein antibody -
Description
Rabbit polyclonal to H1N1 Influenza A virus Nucleocapsid protein -
Host species
Rabbit -
Tested applications
Suitable for: WB, ELISA, ICC/IFmore details -
Species reactivity
Reacts with: Influenza A -
Immunogen
Synthetic peptide derived from internal part of H1N1 Influenza A virus Nucleocapsid protein.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
Constituent: Whole serum -
Concentration information loading...
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Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab104870 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 56 kDa.
Dilute 1:200 - 1:2000 |
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ELISA |
Use at an assay dependent concentration.
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ICC/IF |
Use at an assay dependent concentration.
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Notes |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 56 kDa. Dilute 1:200 - 1:2000 |
ELISA
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
Target
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Relevance
Encapsidates the negative strand viral RNA, protecting it from nucleases. The encapsidated genomic RNA is termed the ribonucleoprotein (RNP) and serves as template for transcription and replication. The RNP needs to be localized in the nucleus to start an infectious cycle, but is too large to diffuse through the nuclear pore complex. NP comprises at least 2 nuclear localization signals and is responsible of the active RNP import into the nucleus through the cellular importin alpha/beta pathway. Later in the infection, nucleus export of RNP are mediated through viral proteins NEP interacting with M1 which binds nucleoproteins. It is possible that the nucleoprotein binds directly exportin-1 (XPO1) and plays an active role in RNP nuclear export. M1 interaction with RNP seems to hide nucleoprotein's nuclear localization signals. Soon after a virion infects a new cell, M1 dissociates from the RNP under acidification of the virion driven by M2 protein. Dissociation of M1 from RNP unmask nucleoprotein's nuclear localization signals, targeting the RNP to the nucleus. -
Cellular localization
Virion. Host nucleus -
Database links
- SwissProt: P03466 Influenza A
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Alternative names
- NP antibody
- Nucleocapsid protein antibody
- Nucleoprotein antibody
- Protein N antibody
Protocols
Datasheets and documents
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Datasheet download
References (6)
ab104870 has been referenced in 6 publications.
- Liu Y et al. ILDR1 promotes influenza A virus replication through binding to PLSCR1. Sci Rep 12:8515 (2022). PubMed: 35595813
- Ren L et al. Influenza A Virus (H1N1) Infection Induces Glycolysis to Facilitate Viral Replication. Virol Sin 36:1532-1542 (2021). PubMed: 34519916
- Yuan S et al. Viruses harness YxxØ motif to interact with host AP2M1 for replication: A vulnerable broad-spectrum antiviral target. Sci Adv 6:eaba7910 (2020). PubMed: 32923629
- Menikou S et al. A proteomics-based method for identifying antigens within immune complexes. PLoS One 15:e0244157 (2020). PubMed: 33362259
- Ren L et al. Influenza A virus (H1N1) triggers a hypoxic response by stabilizing hypoxia-inducible factor-1a via inhibition of proteasome. Virology 530:51-58 (2019). PubMed: 30780125
- Švancarová P & Betáková T Conserved methionine 165 of matrix protein contributes to the nuclear import and is essential for influenza A virus replication. Virol J 15:187 (2018). PubMed: 30509291