Anti-H4R antibody (ab188978)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-H4R antibody
See all H4R primary antibodies -
Description
Rabbit polyclonal to H4R -
Host species
Rabbit -
Specificity
BLAST analysis of the peptide immunogen showed no homology with other human proteins. -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rabbit, Horse, Cow, Dog, Monkey, Common marmoset
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Immunogen
Synthetic peptide within Human H4R. The exact sequence is proprietary. A 18 amino acid peptide from 1st cytoplasmic domain of Human H4R.
Database link: Q9H3N8 -
Positive control
- Human lymph node tissue
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General notes
Previously labelled as HRH4.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab188978 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use a concentration of 12 - 24 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
The H4 subclass of histamine receptors could mediate the histamine signals in peripheral tissues. Displays a significant level of constitutive activity (spontaneous activity in the absence of agonist). -
Tissue specificity
Expressed primarily in the bone marrow and eosinophils. Shows preferential distribution in cells of immunological relevance such as T-cells, dendritic cells, monocytes, mast cells, neutrophils. Also expressed in a wide variety of peripheral tissues, including the heart, kidney, liver, lung, pancreas, skeletal muscle, prostate, small intestine, spleen, testis, colon, fetal liver and lymph node. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 59340 Human
- Entrez Gene: 225192 Mouse
- Omim: 606792 Human
- SwissProt: Q9H3N8 Human
- SwissProt: Q91ZY2 Mouse
- Unigene: 287388 Human
- Unigene: 207073 Mouse
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Alternative names
- AXOR35 antibody
- BG26 antibody
- G protein coupled receptor 105 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-H4R antibody (ab188978)Immunohistochemical analysis of formalin-fixed paraffin-embedded Human lymph node tissue labeling H4R with ab188978 at 24 µg/ml.
Protocols
References
This product has been referenced in:
- Wechsler JB et al. Histamine drives severity of innate inflammation via histamine 4 receptor in murine experimental colitis. Mucosal Immunol 11:861-870 (2018). Read more (PubMed: 29363669) »
