Overview

  • Product name

    Anti-H5 (H5N1) antibody [8D2]
  • Description

    Mouse monoclonal [8D2] to H5 (H5N1)
  • Host species

    Mouse
  • Tested applications

    Suitable for: ELISA, Dot blot, Inhibition Assaymore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Purified avian Influenza Virus H5N1

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • Purity

    Ascites
  • Purification notes

    Purified from ascites.
  • Clonality

    Monoclonal
  • Clone number

    8D2
  • Myeloma

    Sp2/0
  • Isotype

    IgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab82455 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.
Dot blot Use at an assay dependent dilution.
Inhibition Assay Use at an assay dependent dilution.

Target

  • Relevance

    Influenza A virus is a major public health threat. Novel influenza virus strains caused by genetic drift and viral recombination emerge periodically to which humans have little or no immunity, resulting in devastating pandemics. Influenza A can exist in a variety of animals; however it is in birds that all subtypes can be found. These subtypes are classified based on the combination of the virus coat glycoproteins hemagglutinin (HA) and neuraminidase (NA) subtypes. During 1997, an H5N1 avian influenza virus was determined to be the cause of death in 6 of 18 infected patients in Hong Kong. There was some evidence of human to human spread of this virus, but it is thought that the transmission efficiency was fairly low. HA interacts with cell surface proteins containing oligosaccharides with terminal sialyl residues. Virus isolated from a human infected with the H5N1 strain in 1997 could bind to oligosaccharides from human as well as avian sources, indicating its species jumping ability. H5 stands for the fifth of several known types of the viral protein hemagglutinin. This protein binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell. It plays a major role in the determination of host range restriction and virulence and is responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane.
  • Cellular localization

    Virion membrane
  • Alternative names

    • H5N1 haemagglutinin 5 antibody
    • H5N1 hemagglutinin 5 antibody
    • Haemagglutinin antibody
    • Hemagglutinin 5 antibody
    • Hemagglutinin antibody
    • Influenza a antibody
    • Influenza virus haemagglutinin 5 antibody
    • Influenza virus hemagglutinin antibody
    see all

References

This product has been referenced in:

  • Hussain S  et al. Mutation of Influenza A Virus PA-X Decreases Pathogenicity in Chicken Embryos and Can Increase the Yield of Reassortant Candidate Vaccine Viruses. J Virol 93:N/A (2019). Read more (PubMed: 30381488) »
  • Le TT  et al. Dual Recognition Element Lateral Flow Assay Toward Multiplex Strain Specific Influenza Virus Detection. Anal Chem 89:6781-6786 (2017). Read more (PubMed: 28558471) »
See all 3 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Answer

Thank you for contacting us and your interest in our products.

I have the following answers to your questions:

Q1. Is ab82455 https://www.abcam.com/H5-H5N1-antibody-8D2-ab82455.htmlhttps://www.abcam.com/H5-H5N1-antibody-8D2-ab82455.html specific bind to ab123220 (Influenza A, Avian, Vietnam 1203/04, H5N1, Hemagglutinin protein)?

The antibody ab82455 is specific to haemagglutinin H5 of influenza A. It has been tested with following strains:




Antigen
8D2

A/Chicken/Indonesia/Wates1/2005 (H5N1)
Neg

A/Chicken/Indonesia/Wates130/2005 (H5N1)
Neg

A/Duck/Vietnam/942B/2004 (H5N1)
Pos

A/Vietnam/1194/2004 (H5N1)
Pos

A/Vietnam/1203/2004 (H5N1)
Pos

A/Vietnam/1204/2004 (H5N1)
Pos

A/Hong Kong/213/03 (H5N1)
Pos

A/Hong Kong/156/97 (H5N1)
Pos

A/New Caledonia/20/99 (H1N1)
Neg

A/Wyoming/03/2003 (H3N2)
Neg

A/Hong Kong/1073/99 (H9N2)
Neg




The antibody has also been tested with recombinant hemagglutinins H1-H4 and H6-H15 and results were negative with all of them.

Q2. will ab82455 only specific bind to ab123220 (H5N1) but it won't specific bind to ab61986/ab69741 (H1N1) or ab824554/ab124562 (H3N2)?

Following on from the information above, the antibody ab82455 should not cross react with the H1N1 or H3N2 influenza proteins.

Q3. In data sheet of ab135382, it is mentioned "reacts with Human". May I ask that what is the safety requirement for using this item in a Biosafety level 2 environment? is it dangorious to human?

Thank you for pointing this out to us. This is an error. Ab135382 reacts with the viral protein and the reactivity should therefore not have been set to "human". This has now been corrected. You should not need any additional safety measures to use.

Q4. What is the different between ab135382/ab82455/ab73453, if ab123220 is used as the binding pair.

ab135382, ab82455 and ab73453 are all mouse monoclonals raised against the Influenza A virus (H5N1/HA1). The difference comes from the immunogen used to raise the particular antibody. All three should be able to recognise the ab123220. However, as we have not tested any of these products in gold sensor surface testing we would not be able to guarantee how the products would perform.

I hope this information has sufficiently answered your questions, please do not hesitate to ask if you have any further questions.

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Answer

Thank you for contacting us.

Q1. Is ab82455 Anti-H5 (H5N1) antibody [8D2] specific bind to ab123220 (Influenza A, Avian, Vietnam 1203/04, H5N1, Hemagglutinin protein)?

Answer: Yes, the antibody will recognize this protein, ab123220.


Q2. will ab82455 only specific bind to ab123220 (H5N1) but it won't specific bind to ab61986/ab69741 (H1N1) or ab824554/ab124562 (H3N2)?

Answer: Yes, the antibody will only recognize H5N1. The antibody has been tested with recombinant hemagglutinins H1-H4 and H6-H15 and results were negative.


Q3. In data sheet of ab135382, it is mentioned "reacts with Human". May I ask that what is the safety requirement for using this item in a Biosafety level 2 enviroment? is it dangorious to human?

Answer: The antibody is not dangerous. The datasheet note, "Reacts with Human", is a mistake and will be removed from the datasheet. The antibody recognizes a viral protein, not a human protein.


Q4. What is the different between ab135382/ab82455/ab73453, if ab123220 is used as the binding pair.

Answer: Antibody ab82455 is the best choice of the three, since it has been characterized more thoroughly than the other two, and is known to react with Influenza A, Avian, Vietnam 1203/04, H5N1.


I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Answer

Thank you for your enquiry.

I have contacted the originators of these antibodies, who have kindly provided the following testing results from various Influenza A virus strains with these antibodies:

ab59166 Influenza A Virus Hemagglutinin H5 antibody [15A6]

According to the originator, this clone is reactive to strains which express H5 (ie H5N1, H5N2, H5N9). It is not reactive to any other strains that do not express the H5 antigen.

ab73453 Influenza A Virus Hemagglutinin H5 antibody [B253M]

This antibody tested negative with recombinant H1, H3, H7, H9

ab73454 Influenza A Virus Hemagglutinin H5 antibody [B254M]

Has been tested on the following strains:

H5: A/Vietnam/1203/04 (positive)
H5: A/HK/156/97 (positive)
H5N2 (Venice) (positive)
H5N9 (USA (positive)
H5/Clade 2 isolate Far East (negative)
H5: A/HK/213/03 (negative)
H1: A/New Caledonia/20/99 (negative)
H1: A/duck/Alberta/35/78 (negative)
H1: A/sw/Iowa/30 (negative)
H1: A/USSR90/77 (negative)
H3: A/Wyoming/03/2003 (H3) (negative)
H3: A/Equine 2 /Miami/63 (negative)
H3: A/duck/Ukraine/63 (negative)
H7: FPV (negative)
H8: A/turkey/Ontario/61/81/68 (negative)
H9: A/HK/107/99 (H9) (negative)
H9: A/turkey/Wiconsin/66 (negative)
H10: A/chicken/Germany/N/49 (negative)
H11: A/duck/England/56 (negative)
H12: A/duck/Alberta /68/76 (negative)
H13: A/gull/Turkmenia/13/77(negative)

ab73456 Influenza A Virus Hemagglutinin H5 antibody [B256M]

Has been tested on the following strains:

H5: A/Vietnam/1203/04 (positive)
H5: A/HK/156/97 (positive)
H5N2 (Venice) (positive)
H5N9 (USA (positive)
H5/Clade 2 isolate Far East (negative)
H5: A/HK/213/03 (negative)
H1: A/New Caledonia/20/99 (negative)
H1: A/duck/Alberta/35/78 (negative)
H1: A/sw/Iowa/30 (negative)
H1: A/USSR90/77 (negative)
H3: A/Wyoming/03/2003 (H3) (negative)
H3: A/Equine 2 /Miami/63 (negative)
H3: A/duck/Ukraine/63 (negative)
H7: FPV (negative)
H8: A/turkey/Ontario/61/81/68 (negative)
H9: A/HK/107/99 (H9) (negative)
H9: A/turkey/Wiconsin/66 (negative)
H10: A/chicken/Germany/N/49 (negative)
H11: A/duck/England/56 (negative)
H12: A/duck/Alberta /68/76 (negative)
H13: A/gull/Turkmenia/13/77(negative)

ab82455 H5 (H5N1) antibody [8D2]

This antibody is specific to haemagglutinin H5 of influenza A. It has been tested for example with following strains:

A/Chicken/Indonesia/Wates1/2005 (H5N1)Negative
A/Chicken/Indonesia/Wates130/2005 (H5N1) Negative
A/Duck/Vietnam/942B/2004 (H5N1)Positive
A/Vietnam/1194/2004 (H5N1)Positive
A/Vietnam/1203/2004 (H5N1)Positive
A/Vietnam/1204/2004 (H5N1)Positive
A/Hong Kong/213/03 (H5N1)Positive
A/Hong Kong/156/97 (H5N1)Positive
A/New Caledonia/20/99 (H1N1)Negative
A/Wyoming/03/2003 (H3N2)Negative
A/Netherlands/219/03 (H7N7)
A/Hong Kong/1073/99 (H9N2)Negative

It has been tested also with recombinant heamagglutinins H1-H4 and H6-H15 and results were negative with them.

I hope this will be helpful to you. If you have any further questions, please do not hesitate to contact us.

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Answer

Thank you for your inquiry. This antibody will recognize the native and recombinant H5, but we have not tested it on denatured samples. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Question
Answer

Thank you for your inquiry. The immunogen has been received from our academic collaborator and unfortunately due to the confidentiality agreement I am not able to release the strain information. However, it is analog to A/chick/Scotland/72. I hope this information helps. Please contact us with any other questions.

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