Product nameAnti-H6PD/GDH antibody
See all H6PD/GDH primary antibodies
DescriptionRabbit polyclonal to H6PD/GDH
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Synthetic peptide corresponding to Human H6PD/GDH aa 600-700 conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in human liver tissue lysate.
Previously labelled as H6PD.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab72183 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 94 kDa (predicted molecular weight: 88 kDa).|
|ICC/IF||Use a concentration of 5 µg/ml.|
FunctionOxidizes glucose-6-phosphate and glucose, as well as other hexose-6-phosphates.
Tissue specificityPresent in most tissues examined, strongest in liver.
Involvement in diseaseDefects in H6PD are a cause of cortisone reductase deficiency (CRD) [MIM:604931]. In CRD, activation of cortisone to cortisol does not occur, resulting in adrenocorticotropin-mediated androgen excess and a phenotype resembling polycystic ovary syndrome (PCOS).
Sequence similaritiesIn the N-terminal section; belongs to the glucose-6-phosphate dehydrogenase family.
In the C-terminal section; belongs to the glucosamine/galactosamine-6-phosphate isomerase family. 6-phosphogluconolactonase subfamily.
Cellular localizationEndoplasmic reticulum lumen. Microsomes, endoplasmic reticulum lumen.
- Information by UniProt
- 6 phosphogluconolactonase antibody
- 6-phosphogluconolactonase antibody
- 6PGL antibody
Anti-H6PD/GDH antibody (ab72183) at 1 µg/ml + Human liver tissue lysate - total protein (ab29889) at 10 µg
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 88 kDa
Observed band size: 94 kDa why is the actual band size different from the predicted?
Exposure time: 2 minutes
Human GDH/6PGL endoplasmic bifunctional protein precursor (H6PD) has a predicted molecular weight of 88 kDa, however it is glycosylated and phosphorylated which may explain the bands appearing at a higher molecular weight (94 kDa)(SwissProt data). Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
ICC/IF image of ab72183 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab72183, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HepG2, and MCF-7 cells at 5µg/ml.
ab72183 has not yet been referenced specifically in any publications.