Product nameAnti-hSSB1 antibody
DescriptionRabbit polyclonal to hSSB1
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Elephant
Synthetic peptide corresponding to Human hSSB1 aa 161-211.
Database link: NP_076973.1
- HeLa and 293T whole cell lysates.
Previously labelled as OBFC2B.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab85752 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Detects a band of approximately 33 kDa (predicted molecular weight: 23 kDa).|
|IP||Use at 2-5 µg/mg of lysate.|
|IHC-P||1/1000 - 1/5000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Antigen retrieval with Tris-EDTA pH9.0 is recommended for human tissues. Antigen retrieval with citrate buffer pH6.0 is recommended for mouse tissues.
RelevanceSingle-stranded DNA (ssDNA)-binding proteins, such as hSSB1, are ubiquitous and essential for a variety of DNA metabolic processes, including replication, recombination, and detection and repair of damage. hSSB1 plays an important role in DNA damage response. It influences diverse endpoints in the cellular DNA damage response, including cell-cycle checkpoint activation, recombinational repair and maintenance of genomic stability. It associates with DNA lesions, and enables the efficient activation of ATM and consequent phosphorylation of downstream proteins.
Cellular localizationNuclear. Localizes to nuclear foci following DNA damage.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma (left) and mouse squamous cell carcinoma (right) tissues labelling hSSB1 with ab85752 at 1/1000 (1µg/ml). Detection: DAB.
All lanes : Anti-hSSB1 antibody (ab85752) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Predicted band size: 23 kDa
Observed band size: 33 kDa why is the actual band size different from the predicted?
Additional bands at: 38 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes
Detection of Human hSSB1 by Western Blot of Immunprecipitate.
All lanes: ab85752 at 1µg/ml detecting by WB hSSB1 in HeLa whole cell lysates, that were immunoprecipitated with ab85752 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Lane 1: ab85752 Lane 2: Control IgG
Detection: Chemiluminescence with exposure time of 5 seconds.