Anti-hSSB1 antibody (ab85752)
Key features and details
- Rabbit polyclonal to hSSB1
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-hSSB1 antibody -
Description
Rabbit polyclonal to hSSB1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Elephant -
Immunogen
Synthetic peptide corresponding to Human hSSB1 aa 161-211.
Database link: NP_076973.1 -
Positive control
- HeLa and 293T whole cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab85752 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000. Detects a band of approximately 33 kDa (predicted molecular weight: 23 kDa).
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IP |
Use at 2-5 µg/mg of lysate.
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IHC-P |
1/1000 - 1/5000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Antigen retrieval with Tris-EDTA pH9.0 is recommended for human tissues. Antigen retrieval with citrate buffer pH6.0 is recommended for mouse tissues. |
Notes |
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WB
1/2000 - 1/10000. Detects a band of approximately 33 kDa (predicted molecular weight: 23 kDa). |
IP
Use at 2-5 µg/mg of lysate. |
IHC-P
1/1000 - 1/5000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Antigen retrieval with Tris-EDTA pH9.0 is recommended for human tissues. Antigen retrieval with citrate buffer pH6.0 is recommended for mouse tissues. |
Target
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Relevance
Single-stranded DNA (ssDNA)-binding proteins, such as hSSB1, are ubiquitous and essential for a variety of DNA metabolic processes, including replication, recombination, and detection and repair of damage. hSSB1 plays an important role in DNA damage response. It influences diverse endpoints in the cellular DNA damage response, including cell-cycle checkpoint activation, recombinational repair and maintenance of genomic stability. It associates with DNA lesions, and enables the efficient activation of ATM and consequent phosphorylation of downstream proteins. -
Cellular localization
Nuclear. Localizes to nuclear foci following DNA damage. -
Database links
- Entrez Gene: 79035 Human
- Entrez Gene: 69917 Mouse
- Entrez Gene: 362813 Rat
- Omim: 612104 Human
- SwissProt: Q9BQ15 Human
- SwissProt: Q8R2Y9 Mouse
- SwissProt: Q3SWT1 Rat
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Alternative names
- 2610036N15Rik antibody
- hSSB1 antibody
- LP3587 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma (left) and mouse squamous cell carcinoma (right) tissues labelling hSSB1 with ab85752 at 1/1000 (1µg/ml). Detection: DAB.
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All lanes : Anti-hSSB1 antibody (ab85752) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Predicted band size: 23 kDa
Observed band size: 33 kDa why is the actual band size different from the predicted?
Additional bands at: 38 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes -
Detection of Human hSSB1 by Western Blot of Immunprecipitate.
All lanes: ab85752 at 1µg/ml detecting by WB hSSB1 in HeLa whole cell lysates, that were immunoprecipitated with ab85752 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Lane 1: ab85752 Lane 2: Control IgG
Detection: Chemiluminescence with exposure time of 5 seconds.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab85752 has been referenced in 1 publication.
- Chen ZW et al. FBXL5-mediated degradation of single-stranded DNA-binding protein hSSB1 controls DNA damage response. Nucleic Acids Res 42:11560-9 (2014). PubMed: 25249620