Anti-HA tag antibody [12CA5] (ab1424)
![Western blot - Anti-HA tag antibody [12CA5] (ab1424)](https://www.abcam.com/ps/products/1/ab1424/Images/HA-tag-Primary-antibodies-ab1424-6.jpg "Western blot - Anti-HA tag antibody [12CA5] (ab1424)")
Key features and details
- Mouse monoclonal [12CA5] to HA tag
- Suitable for: ICC/IF, ChIP, IP, WB
- Reacts with: Species independent
- Isotype: IgG1
Overview
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Product name
Anti-HA tag antibody [12CA5]
See all HA tag primary antibodies -
Description
Mouse monoclonal [12CA5] to HA tag -
Host species
Mouse -
Specificity
The antibody detects HA-tag fused to the C-terminus or N-terminus of targeted proteins in transfected cells. The antibody works in ChIP but less well than ab9110 (see ChIP data). -
Tested applications
Suitable for: ICC/IF, ChIP, IP, WBmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Synthetic peptide. Derived from the human influenza hemaglutinin protein
Database link: ADG21028 -
Positive control
- WB: Rab6-HA transfected HEK-293T whole cell lysate. IP: Rab6-HA transfected HEK-293T whole cell lysate. ICC/IF: Baby hamster kidney cell expressing HA-tag CFTR protein. ChIP: Xenopus laevis oocytes injected with mRNA for HA-tagged human BORIS.
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General notes
This product is manufactured by BioVision, an Abcam company and was previously called 3996 HA-Tag Antibody (Clone 12CA5[2]).
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.03% Proclin 300
Constituents: 30% Glycerol (glycerin, glycerine), 0.05% BSA, PBS, 0.019% Ethylenediaminetetraacetic acid, disodium, dihydrate -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
12CA5 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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ChIP Related Products
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab1424 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | (1) |
Use a concentration of 10 µg/ml.
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| ChIP |
Use a concentration of 3 µg/ml. Use 3 µg per 25 µg chromatin (see figure legend).
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| IP | (5) |
Use a concentration of 10 µg/ml.
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| WB | (6) |
Use a concentration of 1 µg/ml.
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| Notes |
|---|
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ICC/IF
Use a concentration of 10 µg/ml. |
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ChIP
Use a concentration of 3 µg/ml. Use 3 µg per 25 µg chromatin (see figure legend). |
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IP
Use a concentration of 10 µg/ml. |
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WB
Use a concentration of 1 µg/ml. |
Target
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Relevance
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins. -
Alternative names
- HA epitope tag antibody
- HA1 antibody
- HA2 antibody
see all
Images
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Western blot - Anti-HA tag antibody [12CA5] (ab1424)This image is courtesy of an anonymous AbreviewAnti-HA tag antibody [12CA5] (ab1424) at 1/1000 dilution + HEK293T whole cell lysate over-expressing HA-tagged Rab6 at 20 µg
Secondary
HRP-conjugated goat anti-mouse polyclonal IgG at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 5 seconds
Blocked with 5% milk for 30 min at 25°C -
![Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody [12CA5] (ab1424)](https://www.abcam.com/ps/products/1/ab1424/Images/ab1424_1.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody [12CA5] (ab1424)This image was kindly supplied as part of the review submitted by one of our customers.This picture shows baby hamster kidney cell expressing HA-tag CFTR protein. This type of cells were grown on coverslip and immunofluorescence was performed. Dilution of 1:200 was used and incubated for 2hrs with antibody.
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![Immunoprecipitation - Anti-HA tag antibody [12CA5] (ab1424)](https://www.abcam.com/ps/products/1/ab1424/Images/ab1424-302929-1424.jpg)
Immunoprecipitation - Anti-HA tag antibody [12CA5] (ab1424)Data courtesy of an anonymous AbReviewRecombinant HA-tagged Rab6 was immunoprecipitated from 1 mg transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract with 2 μL ab1424.
Lane 1: Rab6-HA transfected HEK-293T whole cell extract (Input)
Lane 2: ab1424 IP in Rab6-HA transfected HEK-293T whole cell extract.
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![ChIP - Anti-HA tag antibody [12CA5] (ab1424)](https://www.abcam.com/ps/products/1/ab1424/Images/ab1424_2.jpg)
ChIP - Anti-HA tag antibody [12CA5] (ab1424)Xenopus laevis oocytes were injected with mRNA for HA-tagged human BORIS. Chromatin was prepared according to the Abcam X-ChIP protocol. Oocytes were fixed with formaldehyde for 10min. The ChIP was performed with 25µg of chromatin, 3µg of ab1424 (anti-HA, light blue) and 3µg of ab18337 (anti-BORIS, dark blue), and 20µl of Protein A/G sepharose beads. A non-specific antibody was used as a control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (45)
ab1424 has been referenced in 45 publications.
- Melendez J et al. TGFß signalling acts as a molecular brake of myoblast fusion. Nat Commun 12:749 (2021). PubMed: 33531476
- Grigsby SM et al. Elucidating the Importance of DOT1L Recruitment in MLL-AF9 Leukemia and Hematopoiesis. Cancers (Basel) 13:N/A (2021). PubMed: 33562706
- Lei G et al. TRIM29 Reverses Oxaliplatin Resistance of P53 Mutant Colon Cancer Cell. Can J Gastroenterol Hepatol 2021:8870907 (2021). PubMed: 33824865
- Shorstova T et al. Reprogramming of Nucleotide Metabolism Mediates Synergy between Epigenetic Therapy and MAP Kinase Inhibition. Mol Cancer Ther 20:64-75 (2021). PubMed: 33087508
- Ma L et al. The m6A reader YTHDC2 inhibits lung adenocarcinoma tumorigenesis by suppressing SLC7A11-dependent antioxidant function. Redox Biol 38:101801 (2020). PubMed: 33232910