Overview

  • Product name
    Anti-HA tag antibody [16B12]
    See all HA tag primary antibodies
  • Description
    Mouse monoclonal [16B12] to HA tag
  • Host species
    Mouse
  • Specificity
    ab130275 recognizes the Influenza Hemagglutinin epitope (YPYDVPDYA). The antibody recognizes HA epitopes located in the middle of protein sequences as well as at the N- or C-terminus.
  • Tested applications
    Suitable for: WB, IP, ICC/IF, IHC-Frmore details
  • Species reactivity
    Reacts with: Species independent
  • Immunogen

    Synthetic peptide corresponding to HA tag.
    Sequence:

    CYPYDVPDYASL

  • Positive control
    • HA tagged proteins

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.03% Thimerosal (merthiolate)
    Constituents: 49% PBS, 50% Glycerol
  • Concentration information loading...
  • Purity
    Ascites
  • Purification notes
    Purified from ascites.
  • Clonality
    Monoclonal
  • Clone number
    16B12
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab130275 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000.
IP 1/150.
ICC/IF 1/1000.
IHC-Fr Use at an assay dependent concentration.

Target

  • Relevance
    Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
  • Alternative names
    • HA epitope tag antibody
    • HA1 antibody
    • HA2 antibody
    • hemagglutinin antibody
    • Hemagglutinin HA1 chain antibody
    • Hemagglutinin HA2 chain antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence staining of ab130275 on methanol fixed CHO cells transfected with an HA-tagged protein at 1 µg/ml. Cells were then incubated with Alexa Fluor® 594 Goat anti-mouse IgG secondary and were counterstained with DAPI (blue).

  • Western blot of Anti-HA tag antibody [16B12] (ab130275). Lane 1: Molecular weight marker; Lane 2: Recombinant Posi-Tag Epitope Tag Protein; Lane 3: HA.11 overexpressing cell lysate; Lane 4: Untransfected cell lysate.

  • ab130275, at 1/1000 dilution, staining HA tag in Porcine aortic endothelial (PAE) cells expressing Human dopamine transporter tagged with HA by Immunofluorescence.

References

This product has been referenced in:
See all 16 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
Yes - 0.5% Triton X-100
Specification
HeLa
Fixative
Paraformaldehyde

Dr. Kirk Mcmanus

Verified customer

Submitted Jul 03 2018

Application
Western blot
Sample
Mouse Cell lysate - whole cell (mouse neural stem cell)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
40 µg
Specification
mouse neural stem cell
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Dr. Robert Kupp

Verified customer

Submitted Jun 12 2018

Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293T)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
10 µg
Treatment
Cells tranfected with empty vector or HA-tagged protein
Specification
HEK293T
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C

Abcam user community

Verified customer

Submitted May 02 2018

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (4-12 Bis tris)
Sample
Human Cell lysate - whole cell (293T)
Specification
293T
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 18°C

Abcam user community

Verified customer

Submitted May 14 2014

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 18°C
Sample
Human Cell (293T)
Specification
293T
Permeabilization
Yes - 0.5% Triton X100
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted May 13 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up