Key features and details
- Mouse monoclonal [16B12] to HA tag (DyLight® 650)
- Suitable for: ICC/IF, Flow Cyt
- Reacts with: Species independent
- Conjugation: DyLight® 650. Ex: 654nm, Em: 673nm
- Isotype: IgG1
Product nameAnti-HA tag antibody [16B12] (DyLight® 650)
See all HA tag primary antibodies
DescriptionMouse monoclonal [16B12] to HA tag (DyLight® 650)
ConjugationDyLight® 650. Ex: 654nm, Em: 673nm
Specificityab117515 recognizes the Influenza hemagglutinin epitope (YPYDVPDYA).
Tested applicationsSuitable for: ICC/IF, Flow Cytmore details
Species reactivityReacts with: Species independent
Conjugated to Dylight® 650 (ratio 1.3:): Visible absorption maxima 652 nm; Emission maximum 672 nm.
Storage instructionsShipped at 4°C. Store at +4°C. The product can be stored for up to 12 months.
Storage bufferpH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 0.58% Sodium chloride, 95% Sodium phosphate
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab117515 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/500 - 1/5000.|
|Flow Cyt||Use at an assay dependent concentration.|
RelevanceHuman influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
- HA epitope tag antibody
- HA1 antibody
- HA2 antibody
ab117515 staining 293T cells expressing a HA-tagged protein (intracellular) by Flow Cytometry. Cells were permeabilized and fixed. The sample was incubated with the primary antibody (1/200) for 30 minutes at 4°C.
Gating Strategy: Gated on FSC-SSC to exclude debris/dead cells.
Grey - unstained cells.
Red - Wild type 293T, stained.
Blue - 293T expressing HA-tagged protein, stained.
ab117515 has been referenced in 2 publications.
- Feng Y et al. LRRC25 Functions as an Inhibitor of NF-?B Signaling Pathway by Promoting p65/RelA for Autophagic Degradation. Sci Rep 7:13448 (2017). PubMed: 29044191
- Greenwood EJ et al. Temporal proteomic analysis of HIV infection reveals remodelling of the host phosphoproteome by lentiviral Vif variants. Elife 5:N/A (2016). PubMed: 27690223