Key features and details
- Mouse monoclonal [16B12] to HA tag (PE)
- Suitable for: Flow Cyt
- Reacts with: Species independent
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG1
Product nameAnti-HA tag antibody [16B12] (PE)
See all HA tag primary antibodies
DescriptionMouse monoclonal [16B12] to HA tag (PE)
ConjugationPE. Ex: 488nm, Em: 575nm
Specificityab72479 recognizes HA epitopes located in the middle of protein sequences as well as at the N- or C-terminus.
Tested applicationsSuitable for: Flow Cytmore details
Species reactivityReacts with: Species independent
Raised against the twelve amino acid peptide CYPYDVPDYASL.
Epitopeab72479 recognizes the influenza hemagglutinin epitope (YPYDVPDYA).
Excitation max. lambda: 565>498 nm Emission max. lambda: 578 nm.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.013% Sodium azide
Constituents: 0.1% BSA, 0.87% Sodium chloride, 1.64% Sodium phosphate
Concentration information loading...
Purification notesSize exclusion chromatography as well as proprietary methods for purifying the final materials were used.
Our Abpromise guarantee covers the use of ab72479 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration.
ab91357 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
RelevanceHuman influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
- HA epitope tag antibody
- HA1 antibody
- HA2 antibody
ab72479 has been referenced in 2 publications.
- Takano T et al. Dectin-1 intracellular domain determines species-specific ligand spectrum by modulating receptor sensitivity. J Biol Chem 292:16933-16941 (2017). PubMed: 28848046
- Yugawa T et al. Noncanonical NOTCH signaling limits self-renewal of human epithelial and induced pluripotent stem cells through ROCK activation. Mol Cell Biol 33:4434-47 (2013). PubMed: 24019071