Product nameAnti-HA tag antibody [2-2.2.14] (Biotin)
See all HA tag primary antibodies
DescriptionMouse monoclonal [2-2.2.14] to HA tag (Biotin)
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Species independent
- E. coli lysate containing a multi-epitope tagged protein.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Do Not Freeze.
Storage bufferPreservative: 0.02% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab173825 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.|
|WB||1/500 - 1/1000. Predicted molecular weight: 1 kDa.|
RelevanceHuman influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
- HA epitope tag antibody
- HA1 antibody
- HA2 antibody
All lanes : Anti-HA tag antibody [2-2.2.14] (Biotin) (ab173825) at 1/1000 dilution
Lane 1 : E. coli lysate containing a multi-epitope tagged protein at 0.25 µg
Lane 2 : E. coli lysate containing a multi-epitope tagged protein at 0.125 µg
Lane 3 : E. coli lysate containing a multi-epitope tagged protein at 0.0599999986588955 µg
Lane 4 : E. coli lysate containing a multi-epitope tagged protein at 0.0299999993294477 µg
Lane 5 : E. coli lysate containing a multi-epitope tagged protein at 0.0149999996647239 µg
All lanes : Streptavidin-HRP at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 1 kDa
Note: Predicted MW is for the HA tag alone.
ab173825 has not yet been referenced specifically in any publications.