Western blot abreview for Anti-HA tag antibody [4C12] - ChIP Grade

Good
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (human nueroblastoma cells)
Loading amount
25 µg
Specification
human nueroblastoma cells
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Dilution
1/2000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBST

Secondary antibody

Name
Non-Abcam antibody was used: HRP rabbit anit-mouse IgG (H+L)
Host species: Mouse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000

Detection

Detection method
ECL (pierce)
Exposure
20 second(s)
Bands
Specific: 75 kDa Non-specific: 70 kDa
Positive control
HA-tagged protein A transfected cell lysate
Negative control
non-transfected cell lysate

Additional data

Additional Notes
arrow indicated overexpressed protein A

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Submitted Jan 28 2011

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