Western blot abreview for Anti-HA tag antibody [4C12] - ChIP Grade

Excellent
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293T)
Loading amount
20 µg
Specification
HEK293T
Gel Running Conditions
Reduced Denaturing (12% Tris-glycine gel)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk in TBS-Tween-20 (0.05%)

Secondary antibody

Name
Non-Abcam antibody was used: Goat-anti mouse HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000

Detection

Detection method
ECL
Exposure
5 second(s)
Bands
Specific: 25 kDa Non-specific: 75 kDa
Positive control
HEK293T cells over-expressing HA-tagged Rab6

Abcam user community

Verified customer

Submitted Sep 17 2012

Sign up