Immunoprecipitation abreview for Anti-HA tag antibody [4C12] - ChIP Grade

Good
Abreviews
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (Retro virus transduction for protein X)
Total protein in input
500 µg
Specification
Retro virus transduction for protein X
Immuno-precipitation step
Protein A/G

Other product details

Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C

Western Blot antibody

Name
Non-Abcam antibody was used: HRP-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000

Additional data

Additional Notes
first line:control
second line: Protein X transducation by retro virus (50kD)
western blotting was performed with anti-protein X (homemade) after IP.

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Submitted Feb 26 2013

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