Western blot abreview for Anti-HA tag antibody [4C12]

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Abreviews
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Myoblast)
Gel Running Conditions
Non-reduced Denaturing (10% gel)
Loading amount
40 µg
Treatment
Plasmid transfection
Specification
Myoblast
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 18°C

Other product details

Incubation time
14 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk in TBST
Dilution
1/1000

Secondary antibody

Dilution
1/3000
Name
Non-Abcam antibody was used: Amersham ECL Mouse IgG, HRP-linked whole Ab
Host species: Sheep
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL+
Negative control
Non-transfected cells
Exposure
30 second(s)
Bands
Specific: 65 kDa
Positive control
Overexpression HA-tagged fusion protein is 65kDa.

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Submitted Jan 28 2016

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