Western blot abreview for Anti-HA tag antibody [4C12] - ChIP Grade

Good
Abreviews
Application
Western blot
Sample
Mouse Cell lysate - whole cell (HEK293)
Loading amount
10 µg
Specification
HEK293
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C

Other product details

Dilution
1/1000
Incubation time
2 hour(s) and 0 minute(s) · Diluent: 2% milk

Secondary antibody

Name
Non-Abcam antibody was used: rabbit anti mouse
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000

Detection

Detection method
ECL
Exposure
1 hour(s), 0 minute(s) and 0 second(s)
Bands
Specific: 42 kDa
Positive control
Protein tagged with HA
Negative control
non transfected cells

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Submitted Jan 18 2008

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