Overview

  • Product name

  • Description

    Chicken polyclonal to HA tag
  • Host species

    Chicken
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Immunogen

    Synthetic peptide, corresponding to (

    YPYDVPDYA

    ) HA tag conjugated to KLH.

  • General notes

    For longer storage periods, dilute the antibody with sterile glycerol and store at -20°C. Do not freeze unless you want to store for longer periods of time.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab134028 was purified from hyperimmunized hen egg yolks using a proprietary method that yields a purity of >90%. Antibodies were affinity-purified over a peptide column and then dialyzed against phosphate-buffered isotonic saline.
  • Clonality

    Polyclonal
  • Isotype

    IgY
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab134028 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500.
WB 1/5000.

Target

  • Relevance

    Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
  • Alternative names

    • HA epitope tag antibody
    • HA1 antibody
    • HA2 antibody
    • hemagglutinin antibody
    • Hemagglutinin HA1 chain antibody
    • Hemagglutinin HA2 chain antibody
    see all

Images

  • All lanes : Anti-HA tag antibody (ab134028)

    Lane 1 : Cultured COS cells were transfected with a plasmid containing the SAP1a cDNA fused with an HA epitope tag at its C-terminus.
    Lane 2 : Cultured COS cells were transfected with an empty plasmid.

    Lysates/proteins at 25 µg per lane.

References

ab134028 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab134028.
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