Overview

  • Product name

  • Description

    Rabbit polyclonal to HA tag
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IPmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    A synthetic peptide from influenza hemagglutinin epitope: YPYDVPDYA.

  • Positive control

    • HA-recombinant protein transfected HeLa cells; HA-tagged protein.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin
    Constituents: PBS, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab137838 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000.
ICC/IF 1/100 - 1/1000.
IP 1/500.

Target

  • Relevance

    Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
  • Alternative names

    • HA epitope tag antibody
    • HA1 antibody
    • HA2 antibody
    • hemagglutinin antibody
    • Hemagglutinin HA1 chain antibody
    • Hemagglutinin HA2 chain antibody
    see all

Images

  • All lanes : Anti-HA tag antibody (ab137838) at 1/2000 dilution

    Lane 1 : Cell extracts from non-transfected cell line
    Lane 2 : Cell extracts from cell line transfected for HA-tagged protein


    12% SDS PAGE
  • Immunofluorescence analysis of transfected paraformaldehyde-fixed HeLa cells, labelling HA-recombinant protein using ab137838 antibody at 1/500 dilution. Right panel costained with Hoechst 33342.
  • HA-tagged protein was immunoprecipitated from transfected cells using 5 ug of ab137838. The precipitated HA-tagged protein was detected using ab137838 diluted at 1/1000.

References

This product has been referenced in:

  • Ruiz-Llorente L  et al. Characterization of a mutation in the zona pellucida module of Endoglin that causes Hereditary Hemorrhagic Telangiectasia. Gene 696:33-39 (2019). Read more (PubMed: 30763665) »
  • Wei Y  et al. Activation of KRas-ERK1/2 signaling drives the initiation and progression of glioma by suppressing the acetylation of histone H4 at lysine 16. Life Sci 225:55-63 (2019). Read more (PubMed: 30946839) »
See all 9 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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