Overview

  • Product name
  • Description
    Rabbit polyclonal to HA tag
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Immunogen

    Synthetic peptide within HA tag conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    (Peptide available as ab13835)

  • Positive control
    • WB: HEK-293 (Human) Whole Cell Lysate - expressing HA tag protein.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab13834 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Can be blocked with HA tag peptide (ab13835).

Target

  • Relevance
    Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
  • Alternative names
    • HA epitope tag antibody
    • HA1 antibody
    • HA2 antibody
    • hemagglutinin antibody
    • Hemagglutinin HA1 chain antibody
    • Hemagglutinin HA2 chain antibody
    see all

Images

  • All lanes : Anti-HA tag antibody (ab13834) at 1/500 dilution

    Lane 1 : HEK293 (Human) Whole Cell Lysate - expressing HA tag protein (ab20896)
    Lane 2 : HEK293 (Human) Whole Cell Lysate - expressing HA tag protein (ab20896) with HA tag peptide (ab13835) at 1 µg/ml

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Alexa Fluor 680 Goat-anti-Rabbit IgG at 1/5000 dilution

    Performed under reducing conditions.

References

This product has been referenced in:
  • Myers SA  et al. SOX2 O-GlcNAcylation alters its protein-protein interactions and genomic occupancy to modulate gene expression in pluripotent cells. Elife 5:e10647 (2016). Read more (PubMed: 26949256) »
  • Chen X  et al. Deficiency or inhibition of lysophosphatidic acid receptor 1 protects against hyperoxia-induced lung injury in neonatal rats. Acta Physiol (Oxf) 216:358-75 (2016). Read more (PubMed: 26495902) »
See all 6 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Application
IHC - Wholemount
Sample
Mouse Tissue (Brain)
Specification
Brain

Prof. Vidita Vaidya

Verified customer

Submitted Jul 23 2014

Question

Hi Technical,



We have a customer who is looking for a HA tag antibody and a matching secondary, I have sourced and found him the following;



https://www.abcam.com/HA-tag-antibody-ChIP-Grade-ab9110.html

https://www.abcam.com/HA-tag-antibody-ab13834.html

https://www.abcam.com/HA-tag-antibody-Agarose-ab27029.html

https://www.abcam.com/HA-tag-antibody-ChIP-Grade-ab71113.html

https://www.abcam.com/Proteinase-K-ab64220.html



The customer’s first email;

I received your email address from a colleagues at the school of biology sciences. I am looking for some things that you probably sell. First of all an rabbit anti-HA antibody and an anti-rabbit FITC-conjugated antibody (I think they are both from Abcam). And secondly proteinase K. Do you sell these and can you give me a quotation for each of them?



But as he is new to this, he has provide the following details, as he didn’t provide much in the first place.

The customer’s Reply;

Since I’m relatively new to this field of research (I do more behaviour than immunohistochemistry), I’m not entirely sure which of the antibodies is the best. However, perhaps I can explain the experiment and you might know better . We are currently transfecting HEK cells with a mutant receptor that has an HA tag and we want to see whether the receptor is nicely incorporated into the membrane or whether it is somewhere else (using confocal laser microscopy). So we need a good anti-HA antibody for that (and indeed the anti-rabbit conjugated antibody would then be the secondary antibody. I hope that help.

Are you able to help me out with this, as it is out my field?



Looking forward to your response.





Kind regards,



Melissa Gliddon

Customer Service and Technical Support Manager | mailto:melg@sapphirebioscience.com

________________________

Sapphire Bioscience Pty. Ltd.

126 Cope Street │ Waterloo NSW 2017 │ Australia

Ph: +61 2 9698 2022 │ Fax: +61 2 9698 1022 | http://www.sapphirebioscience.com/

Read More
Answer

Thank you for your enquiry, I am pleased to help.

From what I understand here, the customer would liketo detect the HA tag on their recombinant protein expressed in cell culture. This is Immunocytochemistry (ICC). I have reviewed the selection you made:

ab9110 Rabbit polyclonal tested ICC-IF. This will be suitable

ab13834 Rabbit polyclonal tested WB not ICC.This one willnot be guaranteed in ICC-IF for their experiments.

ab27029 - this is conjuated to agarose beads for IP so will not be suitable.

ab71113 - Rabbit polyclonal tested ICC-IF. This will be suitable.

Proteinase K:
Could you confirm for what reason do they need proteinase K? Antigen retrieval is not usually required in ICC and I would suggest to try it only if there is no staining in order to help expose the antigen. In ICC, Fixation should be in just 4% PFA for 10 minutes. Antigen retrieval is usually used for IHC on paraffin embedded sections which are fixed in 10% PFA for at least 24 hours.

There are many suitable secondaries in the catalog. Here are two exmaples of anti-rabbit secondaries tested in ICC-IF conjugated to FITC as requested by the customer:

ab97050 Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (FITC)
Tested in: Flow Cyt, ICC/IF, IHC-P
https://www.abcam.com/index.html?datasheet=97050 (or use the following: https://www.abcam.com/index.html?datasheet=97050).

ab97199Goat polyclonal Secondary Antibody to Rabbit IgG - Fc (FITC)
Tested in: Flow Cyt, ICC/IF, IHC-P
https://www.abcam.com/index.html?datasheet=97199 (or use the following: https://www.abcam.com/index.html?datasheet=97199).

We also have an ICC webinar coming soon which they may like to consider reviewing. This is being held next week, regrettably not at a convenient time for Australia. However, a copy will be available on our blog (link at the bottom of the Abcam homepage)in early May.

The following link aboutthis webinaralso contains links to some ICC protocols (bottom left hand side)which I hope will be helpful:
https://www.abcam.com/index.html?pageconfig=resource&rid=14548

I hope this will be useful. If you have any further questions, please do not hesitate to contact me.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa cells)
Loading amount
40 µg
Specification
HeLa cells
Gel Running Conditions
Reduced Denaturing (10% Bis-Tris)
Blocking step
LI-COR® Odyssey® Blocking Buffer as blocking agent for 45 minute(s) · Concentration: 50% · Temperature: RT°C

Dr. Svetlana Khoronenkova

Verified customer

Submitted Aug 17 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Human glioma cells)
Loading amount
250000 cells
Specification
Human glioma cells
Treatment
Transfected with lipofectamine
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Sep 04 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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