Anti-HA tag antibody (ab184643)
Key features and details
- Rabbit polyclonal to HA tag
- Suitable for: WB, IP, ICC, ELISA
- Reacts with: Species independent
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-HA tag antibody
See all HA tag primary antibodies -
Description
Rabbit polyclonal to HA tag -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, ICC, ELISAmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Synthetic peptide conjugated to keyhole limpet haemocyanin.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8 -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab184643 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/30000.
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| IP |
Use a concentration of 1 - 4 µg/ml.
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| ICC |
1/100 - 1/400.
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| ELISA |
1/1000 - 1/30000.
for coating plates use a dilution range of 1/1000 - 1/30,000 |
| Notes |
|---|
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WB
1/1000 - 1/30000. |
|
IP
Use a concentration of 1 - 4 µg/ml. |
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ICC
1/100 - 1/400. |
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ELISA
1/1000 - 1/30000. for coating plates use a dilution range of 1/1000 - 1/30,000 |
Target
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Relevance
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins. -
Alternative names
- HA epitope tag antibody
- HA1 antibody
- HA2 antibody
see all
Images
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Western blot - Anti-HA tag antibody (ab184643)
Detection of HA-tagged Protein by Western Blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody: Affinity purified rabbit anti-HA tag antibody ab184643 used for WB at 0.04 µg/ml (1:25,000). Detection: Chemiluminescence with an exposure time of 3 seconds.
Protocols
Datasheets and documents
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Datasheet download
References (1)
ab184643 has been referenced in 1 publication.
- Adamczyk M & Szatkowska R Low RNA Polymerase III activity results in up regulation of HXT2 glucose transporter independently of glucose signaling and despite changing environment. PLoS One 12:e0185516 (2017). PubMed: 28961268
