Key features and details
- Rabbit polyclonal to HA tag
- Suitable for: WB, IP, ICC, ELISA
- Reacts with: Species independent
- Isotype: IgG
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Concentration information loading...
Our Abpromise guarantee covers the use of ab184643 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/30000.|
|IP||Use a concentration of 1 - 4 µg/ml.|
|ICC||1/100 - 1/400.|
|ELISA||1/1000 - 1/30000.
for coating plates use a dilution range of 1/1000 - 1/30,000
RelevanceHuman influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
- HA epitope tag antibody
- HA1 antibody
- HA2 antibody
Detection of HA-tagged Protein by Western Blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody: Affinity purified rabbit anti-HA tag antibody ab184643 used for WB at 0.04 µg/ml (1:25,000). Detection: Chemiluminescence with an exposure time of 3 seconds.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab184643 has been referenced in 1 publication.
- Adamczyk M & Szatkowska R Low RNA Polymerase III activity results in up regulation of HXT2 glucose transporter independently of glucose signaling and despite changing environment. PLoS One 12:e0185516 (2017). PubMed: 28961268