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    ha-tag-antibody-ab184643.pdf

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Tags & Cell Markers Epitope Tags HA Tag
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Anti-HA tag antibody (ab184643)

  • Datasheet
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Western blot - Anti-HA tag antibody (ab184643)

    Key features and details

    • Rabbit polyclonal to HA tag
    • Suitable for: WB, IP, ICC, ELISA
    • Reacts with: Species independent
    • Isotype: IgG

    Get better batch-to-batch reproducibility with a recombinant antibody

    Product image
    Anti-HA tag antibody [EPR22819-101] (ab236632)
    • Research with confidence – consistent and reproducible results with every batch
    • Long-term and scalable supply – powered by recombinant technology for fast production
    • Success from the first experiment – confirmed specificity through extensive validation
    • Ethical standards compliant – production is animal-free

    Overview

    • Product name

      Anti-HA tag antibody
      See all HA tag primary antibodies
    • Description

      Rabbit polyclonal to HA tag
    • Host species

      Rabbit
    • Tested applications

      Suitable for: WB, IP, ICC, ELISAmore details
    • Species reactivity

      Reacts with: Species independent
    • Immunogen

      Synthetic peptide conjugated to keyhole limpet haemocyanin.

      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 6.8
    • Concentration information loading...
    • Purity

      Affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Tags & Cell Markers
      • Epitope Tags
      • HA Tag

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab184643 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB
    1/1000 - 1/30000.
    IP
    Use a concentration of 1 - 4 µg/ml.
    ICC
    1/100 - 1/400.
    ELISA
    1/1000 - 1/30000.

    for coating plates use a dilution range of 1/1000 - 1/30,000

    Notes
    WB
    1/1000 - 1/30000.
    IP
    Use a concentration of 1 - 4 µg/ml.
    ICC
    1/100 - 1/400.
    ELISA
    1/1000 - 1/30000.

    for coating plates use a dilution range of 1/1000 - 1/30,000

    Target

    • Relevance

      Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
    • Alternative names

      • HA epitope tag antibody
      • HA1 antibody
      • HA2 antibody
      • hemagglutinin antibody
      • Hemagglutinin HA1 chain antibody
      • Hemagglutinin HA2 chain antibody
      see all

    Images

    • Western blot - Anti-HA tag antibody (ab184643)
      Western blot - Anti-HA tag antibody (ab184643)

      Detection of HA-tagged Protein by Western Blot.  Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein.  Antibody: Affinity purified rabbit anti-HA tag antibody ab184643 used for WB at 0.04 µg/ml (1:25,000). Detection: Chemiluminescence with an exposure time of 3 seconds. 

    Protocols

    • Immunoprecipitation protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (1)

    Publishing research using ab184643? Please let us know so that we can cite the reference in this datasheet.

    ab184643 has been referenced in 1 publication.

    • Adamczyk M & Szatkowska R Low RNA Polymerase III activity results in up regulation of HXT2 glucose transporter independently of glucose signaling and despite changing environment. PLoS One 12:e0185516 (2017). PubMed: 28961268

    Customer reviews and Q&As

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