Overview

  • Product name

  • Description

    Chicken polyclonal to HA tag
  • Host species

    Chicken
  • Tested applications

    Suitable for: WB, Flow Cyt, ELISAmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Synthetic peptide corresponding to HA tag conjugated to Keyhole Limpet Haemocyanin (KLH).
    Sequence:

    YPYDVPDYA

  • Positive control

    • Labeled scFv displaying yeast population.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.1% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    After multiple immunizations in Freunds adjuvant, eggs were collected and the immunoglobulin fraction prepared. Antibodies were immunoaffinity purified using the peptide immobilized on a solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgY
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab190108 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500.
Flow Cyt 1/75 - 1/200.

ab37382 - Chicken polyclonal IgY, is suitable for use as an isotype control with this antibody.

 

ELISA Use at an assay dependent concentration.

ab190108 diluted 1/10000 gave an O.D.= 1.0 in a 15 minute reaction against peptide conjugated to a different carrier than used for anti peptide purification.

Target

  • Relevance

    Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
  • Alternative names

    • HA epitope tag antibody
    • HA1 antibody
    • HA2 antibody
    • hemagglutinin antibody
    • Hemagglutinin HA1 chain antibody
    • Hemagglutinin HA2 chain antibody
    see all

Images

  • Flow cytometric analysis of labeled scFv displaying yeast population labeling HA tag with ab190108 at 1/75 or 1/200 dilution, followed by anti-chicken IgY FITC conjugate secondary antibody at 1/100 dilution.

References

ab190108 has not yet been referenced specifically in any publications.

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