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    ha-tag-antibody-ab20084.pdf

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Tags & Cell Markers Epitope Tags HA Tag
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Anti-HA tag antibody (ab20084)

  • Datasheet
  • SDS
Reviews (2) Submit a question References (16)

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Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody (ab20084)

    Key features and details

    • Rabbit polyclonal to HA tag
    • Suitable for: ELISA, ICC/IF, WB
    • Reacts with: Species independent
    • Isotype: IgG

    Get better batch-to-batch reproducibility with a recombinant antibody

    Product image
    Anti-HA tag antibody [EPR22819-101] (ab236632)
    • Research with confidence – consistent and reproducible results with every batch
    • Long-term and scalable supply – powered by recombinant technology for fast production
    • Success from the first experiment – confirmed specificity through extensive validation
    • Ethical standards compliant – production is animal-free

    Overview

    • Product name

      Anti-HA tag antibody
      See all HA tag primary antibodies
    • Description

      Rabbit polyclonal to HA tag
    • Host species

      Rabbit
    • Tested applications

      Suitable for: ELISA, ICC/IF, WBmore details
    • Species reactivity

      Reacts with: Species independent
    • Immunogen

      Synthetic peptide corresponding to HA tag (internal sequence) conjugated to keyhole limpet haemocyanin.
      Sequence:

      YPYDVPDYA

      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • General notes


      The antibody ab20084 is able to recognise the HA epitope when used to tag other proteins.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
    • Storage buffer

      Preservative: 0.1% Sodium azide
      Constituent: PBS
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Primary antibody notes

      The antibody ab20084 is able to recognise the HA epitope when used to tag other proteins.
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Tags & Cell Markers
      • Epitope Tags
      • HA Tag
      • Microbiology
      • Organism
      • Virus
      • RNA Virus
      • single stranded RNA negative strand virus
      • Influenza

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Positive Controls

      • Recombinant Influenza A H3 (H3N2) protein (ab124562)
      • Recombinant Influenza A Virus Hemagglutinin protein (ab124596)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab20084 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    ELISA
    1/70000. The anti HA diluted 1/70,000 gave an OD=1.0 in a 15 minute reaction against peptide conjugated with a different carrier than used for anti peptide purification.
    ICC/IF (1)
    Use at an assay dependent dilution.
    WB (1)
    1/5000. Predicted molecular weight: 1 kDa.
    Notes
    ELISA
    1/70000. The anti HA diluted 1/70,000 gave an OD=1.0 in a 15 minute reaction against peptide conjugated with a different carrier than used for anti peptide purification.
    ICC/IF
    Use at an assay dependent dilution.
    WB
    1/5000. Predicted molecular weight: 1 kDa.

    Target

    • Relevance

      Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
    • Alternative names

      • HA epitope tag antibody
      • HA1 antibody
      • HA2 antibody
      • hemagglutinin antibody
      • Hemagglutinin HA1 chain antibody
      • Hemagglutinin HA2 chain antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody (ab20084)
      Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody (ab20084)Image from Wu CY et al., PLoS One. 2010 Mar 22;5(3):e9784. doi: 10.1371/journal.pone.0009784.; Fig 1.; March 22, 2010, PLoS ONE 5(3): e9784.
      Immunofluorescence analysis of influenza virus-like particle producer cells, staining Hemagglutinin (red) with ab20084.

      Cells were fixed in paraformaldehyde for 10 min and permeabilized in 0.05% Triton-X 100 for 1 min. After blocking with 1% gelatin, the cells were incubated with primary antibody, followed by incubation with Cy3-conjugated goat anti-rabbit IgG as the secondary antibody.

    Protocols

    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (16)

    Publishing research using ab20084? Please let us know so that we can cite the reference in this datasheet.

    ab20084 has been referenced in 16 publications.

    • Gingell JJ  et al. Distinct Patterns of Internalization of Different Calcitonin Gene-Related Peptide Receptors. ACS Pharmacol Transl Sci 3:296-304 (2020). PubMed: 32296769
    • Lulla V & Firth AE A hidden gene in astroviruses encodes a viroporin. Nat Commun 11:4070 (2020). PubMed: 32792502
    • Yoon S  et al. TGF-ß-Induced Phosphorylation of Usp9X Stabilizes Ankyrin-G and Regulates Dendritic Spine Development and Maintenance. Cell Rep 31:107685 (2020). PubMed: 32460012
    • Cheng Z  et al. K-Ras-ERK1/2 accelerates lung cancer cell development via mediating H3K18ac through the MDM2-GCN5-SIRT7 axis. Pharm Biol 57:701-709 (2019). PubMed: 31613681
    • Turi GF  et al. Vasoactive Intestinal Polypeptide-Expressing Interneurons in the Hippocampus Support Goal-Oriented Spatial Learning. Neuron 101:1150-1165.e8 (2019). PubMed: 30713030
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-2 of 2 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Hemagglutinin antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Permeabilization
    Yes - 0.5% Triton
    Specification
    HeLa
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 01 2017

    Western blot abreview for Anti-Hemagglutinin antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HeLa)
    Gel Running Conditions
    Reduced Denaturing (8%)
    Loading amount
    50 µg
    Specification
    HeLa
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jan 23 2017

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