Anti-HA tag antibody (ab20084)
- Datasheet
- References (7)
- Protocols
Overview
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Product nameAnti-HA tag antibody
See all HA tag primary antibodies -
DescriptionRabbit polyclonal to HA tag
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Host speciesRabbit
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Tested applicationsSuitable for: ELISA, ICC/IF, WBmore details
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Species reactivityReacts with: Species independent
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Immunogen
Synthetic peptide corresponding to HA tag (internal sequence) conjugated to keyhole limpet haemocyanin.
Sequence:YPYDVPDYA
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General notes
The antibody ab20084 is able to recognise the HA epitope when used to tag other proteins.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferPreservative: 0.1% Sodium Azide
Constituents: PBS, pH 7.2 -
Concentration information loading... -
PurityImmunogen affinity purified
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Primary antibody notesThe antibody ab20084 is able to recognise the HA epitope when used to tag other proteins.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab20084 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA | 1/70000. The anti HA diluted 1/70,000 gave an OD=1.0 in a 15 minute reaction against peptide conjugated with a different carrier than used for anti peptide purification. | |
| ICC/IF | Use at an assay dependent dilution. | |
| WB | 1/5000. Predicted molecular weight: 1 kDa. |
Target
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RelevanceHuman influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
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Alternative names
- HA epitope tag antibody
- HA1 antibody
- HA2 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody (ab20084)Image from Wu CY et al., PLoS One. 2010 Mar 22;5(3):e9784. doi: 10.1371/journal.pone.0009784.; Fig 1.; March 22, 2010, PLoS ONE 5(3): e9784.Immunofluorescence analysis of influenza virus-like particle producer cells, staining Hemagglutinin (red) with ab20084.
Cells were fixed in paraformaldehyde for 10 min and permeabilized in 0.05% Triton-X 100 for 1 min. After blocking with 1% gelatin, the cells were incubated with primary antibody, followed by incubation with Cy3-conjugated goat anti-rabbit IgG as the secondary antibody.
References
This product has been referenced in:
- Fueyo R et al. Lineage specific transcription factors and epigenetic regulators mediate TGFß-dependent enhancer activation. Nucleic Acids Res N/A:N/A (2018). Read more (PubMed: 29438503) »
- Asensio-Juan E et al. The histone demethylase PHF8 is a molecular safeguard of the IFN? response. Nucleic Acids Res 45:3800-3811 (2017). Read more (PubMed: 28100697) »
