Overview

  • Product name

  • Description

    Rabbit polyclonal to HA tag
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ELISA, ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Synthetic peptide corresponding to HA tag (internal sequence) conjugated to keyhole limpet haemocyanin.
    Sequence:

    YPYDVPDYA

  • General notes


    The antibody ab20084 is able to recognise the HA epitope when used to tag other proteins.

Properties

Applications

Our Abpromise guarantee covers the use of ab20084 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/70000. The anti HA diluted 1/70,000 gave an OD=1.0 in a 15 minute reaction against peptide conjugated with a different carrier than used for anti peptide purification.
ICC/IF Use at an assay dependent dilution.
WB 1/5000. Predicted molecular weight: 1 kDa.

Target

  • Relevance

    Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
  • Alternative names

    • HA epitope tag antibody
    • HA1 antibody
    • HA2 antibody
    • hemagglutinin antibody
    • Hemagglutinin HA1 chain antibody
    • Hemagglutinin HA2 chain antibody
    see all

Images

  • Immunofluorescence analysis of influenza virus-like particle producer cells, staining Hemagglutinin (red) with ab20084.

    Cells were fixed in paraformaldehyde for 10 min and permeabilized in 0.05% Triton-X 100 for 1 min. After blocking with 1% gelatin, the cells were incubated with primary antibody, followed by incubation with Cy3-conjugated goat anti-rabbit IgG as the secondary antibody.

References

This product has been referenced in:

  • Fueyo R  et al. Lineage specific transcription factors and epigenetic regulators mediate TGFß-dependent enhancer activation. Nucleic Acids Res N/A:N/A (2018). Read more (PubMed: 29438503) »
  • Le Dréau G  et al. E proteins sharpen neurogenesis by modulating proneural bHLH transcription factors' activity in an E-box-dependent manner. Elife 7:N/A (2018). Read more (PubMed: 30095408) »
See all 8 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
Yes - 0.5% Triton
Specification
HeLa
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Feb 01 2017

Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa)
Gel Running Conditions
Reduced Denaturing (8%)
Loading amount
50 µg
Specification
HeLa
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jan 23 2017

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