Overview

  • Product name

  • Description

    Goat polyclonal to HA tag
  • Host species

    Goat
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Synthetic peptide corresponding to HA tag conjugated to keyhole limpet haemocyanin.
    Sequence:

    YPYDVPDY

  • Positive control

    • E. coli whole cell lysate, expressing a multi-tag fusion protein.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab215069 was isolated by affinity chromatography using the peptide immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab215069 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/25000.

Target

  • Relevance

    Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
  • Alternative names

    • HA epitope tag antibody
    • HA1 antibody
    • HA2 antibody
    • hemagglutinin antibody
    • Hemagglutinin HA1 chain antibody
    • Hemagglutinin HA2 chain antibody
    see all

Images

  • All lanes : Anti-HA tag antibody (ab215069) at 1/25000 dilution

    Lane 1 : E. coli whole cell lysate expressing a multi-tag fusion protein at 0.2 µg
    Lane 2 : E. coli whole cell lysate expressing a multi-tag fusion protein at 0.067 µg
    Lane 3 : E. coli whole cell lysate expressing a multi-tag fusion protein at 0.022 µg
    Lane 4 : E. coli whole cell lysate expressing a multi-tag fusion protein at 0.007 µg
    Lane 5 : E. coli whole cell lysate expressing a multi-tag fusion protein at 0.002 µg

    Developed using the ECL technique.

    Exposure time: 10 seconds

References

ab215069 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab215069.
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