Anti-HA tag antibody (ab9111)

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Overview

  • Product name
    Anti-HA tag antibody
    See all HA tag primary antibodies
  • Description
    Chicken polyclonal to HA tag
  • Host species
    Chicken
  • Specificity
    ELISA: this antibody diluted at 1:20,000 gave an O.D.= 1.0 in a 15 minute reaction against peptide conjugated to a different carrier than used for anti peptide purification. HRP conjugated goat anti chicken IgG (IgY) was used and TMB was the substrate.
  • Tested applications
    Suitable for: ICC/IF, WB, ELISAmore details
  • Species reactivity
    Reacts with: Species independent
  • Immunogen

    YPYDVPDYA (influenza hemagglutinin-HA (epitope) conjugated to KLH.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.1% Sodium azide
  • Concentration information loading...
  • Purity
    IgY fraction
  • Purification notes
    Antibodies were immunoaffinity purified using the peptide immobilized on a solid support.
  • Clonality
    Polyclonal
  • Isotype
    unknown
  • Light chain type
    unknown
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab9111 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent dilution. PubMed: 20943657
WB 1/1000.
ELISA Use at an assay dependent dilution.

Target

  • Relevance
    Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
  • Alternative names
    • HA epitope tag antibody
    • HA1 antibody
    • HA2 antibody
    • hemagglutinin antibody
    • Hemagglutinin HA1 chain antibody
    • Hemagglutinin HA2 chain antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody (ab9111)
    Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody (ab9111)Image from Velayos-Baeza A et al, J Biol Chem. 2010 Dec 17;285(51):40148-62. Epub 2010 Oct 13, Fig 7. DOI 10.1074/jbc.M110.145961
    ab9111 staining HA tag (red) in 293T cells overexpressing KHAmCt-980 protein by Immunocytochemistry/ Immunofluorescence. Nucleoli stained with DAPI. Green is for specific speckle marker.

References

This product has been referenced in:
See all 7 Publications for this product

Publishing research using ab9111? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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Question

Hello, we are considering tagging out proteins for in vitro translation using an HA tag. Our current plan is to express the tag on the N terminal. We are using a (GGS)3 linker between domains of our protein so I am wondering what I should use for a linker to attach the tag to the N terminal of our protein. Any suggestions, experience or references would be greatly appreciated.

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Answer

Unfortunately our expertise is in the field of antibody production and epitope design. We work closely with a range of collaborators to produce fusion or recombinant proteins and therefore are unable to offer the information that you require. We wish you every success in your research and would be happy to help with antibody related queries in future

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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