Anti-HA tag antibody (ab9111)
Key features and details
- Chicken polyclonal to HA tag
- Suitable for: WB, ELISA
- Reacts with: Species independent
- Isotype: unknown
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-HA tag antibody
See all HA tag primary antibodies -
Description
Chicken polyclonal to HA tag -
Host species
Chicken -
Specificity
ELISA: this antibody diluted at 1:20,000 gave an O.D.= 1.0 in a 15 minute reaction against peptide conjugated to a different carrier than used for anti peptide purification. HRP conjugated goat anti chicken IgG (IgY) was used and TMB was the substrate. -
Tested applications
Suitable for: WB, ELISAmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Synthetic peptide corresponding to Influenza A HA tag conjugated to keyhole limpet haemocyanin. Influenza hemagglutinin-HA (epitope)
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.1% Sodium azide -
Concentration information loading... -
Purity
IgY fraction -
Purification notes
Antibodies were immunoaffinity purified using the peptide immobilized on a solid support. -
Clonality
Polyclonal -
Isotype
unknown -
Light chain type
unknown -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab9111 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000.
|
|
| ELISA |
Use at an assay dependent concentration.
|
| Notes |
|---|
|
WB
1/1000. |
|
ELISA
Use at an assay dependent concentration. |
Target
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Relevance
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins. -
Alternative names
- HA epitope tag antibody
- HA1 antibody
- HA2 antibody
see all
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (15)
ab9111 has been referenced in 15 publications.
- Decombe S et al. Epigenetic rewriting at centromeric DNA repeats leads to increased chromatin accessibility and chromosomal instability. Epigenetics Chromatin 14:35 (2021). PubMed: 34321103
- Lim CKW et al. Treatment of a Mouse Model of ALS by In Vivo Base Editing. Mol Ther 28:1177-1189 (2020). PubMed: 31991108
- Khlghatyan J et al. High Sensitivity Mapping of Cortical Dopamine D2 Receptor Expressing Neurons. Cereb Cortex 29:3813-3827 (2019). PubMed: 30295716
- Golinski AW et al. Biophysical Characterization Platform Informs Protein Scaffold Evolvability. ACS Comb Sci 21:323-335 (2019). PubMed: 30681831
- Fabry MH et al. piRNA-guided co-transcriptional silencing coopts nuclear export factors. Elife 8:N/A (2019). PubMed: 31219034