Anti-HA tag antibody (ab9111)
Key features and details
- Chicken polyclonal to HA tag
- Suitable for: ICC/IF, WB, ELISA
- Reacts with: Species independent
- Isotype: unknown
Overview
-
Product name
Anti-HA tag antibody
See all HA tag primary antibodies -
Description
Chicken polyclonal to HA tag -
Host species
Chicken -
Specificity
ELISA: this antibody diluted at 1:20,000 gave an O.D.= 1.0 in a 15 minute reaction against peptide conjugated to a different carrier than used for anti peptide purification. HRP conjugated goat anti chicken IgG (IgY) was used and TMB was the substrate. -
Tested applications
Suitable for: ICC/IF, WB, ELISAmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
YPYDVPDYA (influenza hemagglutinin-HA (epitope) conjugated to KLH.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.1% Sodium azide -
Concentration information loading... -
Purity
IgY fraction -
Purification notes
Antibodies were immunoaffinity purified using the peptide immobilized on a solid support. -
Clonality
Polyclonal -
Isotype
unknown -
Light chain type
unknown -
Research areas
Applications
Our Abpromise guarantee covers the use of ab9111 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use at an assay dependent dilution. PubMed: 20943657 | |
| WB | 1/1000. | |
| ELISA | Use at an assay dependent dilution. |
Target
-
Relevance
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins. -
Alternative names
- HA epitope tag antibody
- HA1 antibody
- HA2 antibody
see all
Images
-
Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody (ab9111)Image from Velayos-Baeza A et al, J Biol Chem. 2010 Dec 17;285(51):40148-62. Epub 2010 Oct 13, Fig 7. DOI 10.1074/jbc.M110.145961ab9111 staining HA tag (red) in 293T cells overexpressing KHAmCt-980 protein by Immunocytochemistry/ Immunofluorescence. Nucleoli stained with DAPI. Green is for specific speckle marker.
Protocols
References
ab9111 has been referenced in 11 publications.
- Golinski AW et al. Biophysical Characterization Platform Informs Protein Scaffold Evolvability. ACS Comb Sci 21:323-335 (2019). PubMed: 30681831
- Fabry MH et al. piRNA-guided co-transcriptional silencing coopts nuclear export factors. Elife 8:N/A (2019). PubMed: 31219034
- Boone PG et al. A cancer rainbow mouse for visualizing the functional genomics of oncogenic clonal expansion. Nat Commun 10:5490 (2019). PubMed: 31792216
- Ritter SC & Hackel BJ Validation and Stabilization of a Prophage Lysin of Clostridium perfringens by Using Yeast Surface Display and Coevolutionary Models. Appl Environ Microbiol 85:N/A (2019). PubMed: 30850429
- Darling TL et al. Intracellular Crosslinking of Filoviral Nucleoproteins with Xintrabodies Restricts Viral Packaging. Front Immunol 8:1197 (2017). PubMed: 29021793
