Anti-HA tag antibody (ab9111)

Reviews (1)Q&A (1)References (12)

Key features and details

  • Chicken polyclonal to HA tag
  • Suitable for: WB, ELISA
  • Reacts with: Species independent
  • Isotype: unknown

Get better batch-to-batch reproducibility with a recombinant antibody

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Anti-HA tag antibody [EPR4095] (ab182009)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-HA tag antibody
    See all HA tag primary antibodies

  • Description

    Chicken polyclonal to HA tag

  • Host species

    Chicken

  • Specificity

    ELISA: this antibody diluted at 1:20,000 gave an O.D.= 1.0 in a 15 minute reaction against peptide conjugated to a different carrier than used for anti peptide purification. HRP conjugated goat anti chicken IgG (IgY) was used and TMB was the substrate.

  • Tested applications

    Suitable for: WB, ELISAmore details

  • Species reactivity

    Reacts with: Species independent

  • Immunogen

    YPYDVPDYA (influenza hemagglutinin-HA (epitope) conjugated to KLH.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.20
    Preservative: 0.1% Sodium azide
  • Concentration information loading...

  • Purity

    IgY fraction

  • Purification notes

    Antibodies were immunoaffinity purified using the peptide immobilized on a solid support.

  • Clonality

    Polyclonal

  • Isotype

    unknown

  • Light chain type

    unknown

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab9111 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000.
ELISA
Use at an assay dependent concentration.
Notes
WB
1/1000.
ELISA
Use at an assay dependent concentration.

Target

  • Relevance

    Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.

  • Alternative names

    • HA epitope tag antibody
    • HA1 antibody
    • HA2 antibody
    • hemagglutinin antibody
    • Hemagglutinin HA1 chain antibody
    • Hemagglutinin HA2 chain antibody
    see all

References (12)

Publishing research using ab9111? Please let us know so that we can cite the reference in this datasheet.

ab9111 has been referenced in 12 publications.

  • Golinski AW  et al. Biophysical Characterization Platform Informs Protein Scaffold Evolvability. ACS Comb Sci 21:323-335 (2019). PubMed: 30681831
  • Fabry MH  et al. piRNA-guided co-transcriptional silencing coopts nuclear export factors. Elife 8:N/A (2019). PubMed: 31219034
  • Boone PG  et al. A cancer rainbow mouse for visualizing the functional genomics of oncogenic clonal expansion. Nat Commun 10:5490 (2019). PubMed: 31792216
  • Ritter SC & Hackel BJ Validation and Stabilization of a Prophage Lysin of Clostridium perfringens by Using Yeast Surface Display and Coevolutionary Models. Appl Environ Microbiol 85:N/A (2019). PubMed: 30850429
  • Sauerwald J  et al. Matrix metalloproteinase 1 modulates invasive behavior of tracheal branches during entry into Drosophila flight muscles. Elife 8:N/A (2019). PubMed: 31577228
View all Publications for this product

Customer reviews and Q&As

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1-2 of 2 Abreviews or Q&A

Immunohistochemistry free floating abreview for Anti-HA tag antibody

 Below Average
Abreviews
abreview image
Application
Immunohistochemistry free floating
Sample
Mouse Cell (Brain sections - IHCFrFl)
Specification
Brain sections - IHCFrFl
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Abcam user community

Verified customer

Submitted Feb 07 2020

Question

Hello, we are considering tagging out proteins for in vitro translation using an HA tag. Our current plan is to express the tag on the N terminal. We are using a (GGS)3 linker between domains of our protein so I am wondering what I should use for a linker to attach the tag to the N terminal of our protein. Any suggestions, experience or references would be greatly appreciated.

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Answer

Unfortunately our expertise is in the field of antibody production and epitope design. We work closely with a range of collaborators to produce fusion or recombinant proteins and therefore are unable to offer the information that you require. We wish you every success in your research and would be happy to help with antibody related queries in future

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