Key features and details
- Chicken polyclonal to HA tag
- Suitable for: ICC/IF, WB, ELISA
- Reacts with: Species independent
- Isotype: unknown
Product nameAnti-HA tag antibody
See all HA tag primary antibodies
DescriptionChicken polyclonal to HA tag
SpecificityELISA: this antibody diluted at 1:20,000 gave an O.D.= 1.0 in a 15 minute reaction against peptide conjugated to a different carrier than used for anti peptide purification. HRP conjugated goat anti chicken IgG (IgY) was used and TMB was the substrate.
Tested applicationsSuitable for: ICC/IF, WB, ELISAmore details
Species reactivityReacts with: Species independent
YPYDVPDYA (influenza hemagglutinin-HA (epitope) conjugated to KLH.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.1% Sodium azide
Concentration information loading...
Purification notesAntibodies were immunoaffinity purified using the peptide immobilized on a solid support.
Light chain typeunknown
Our Abpromise guarantee covers the use of ab9111 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent dilution. PubMed: 20943657|
|ELISA||Use at an assay dependent dilution.|
RelevanceHuman influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
- HA epitope tag antibody
- HA1 antibody
- HA2 antibody
ab9111 has been referenced in 11 publications.
- Golinski AW et al. Biophysical Characterization Platform Informs Protein Scaffold Evolvability. ACS Comb Sci 21:323-335 (2019). PubMed: 30681831
- Fabry MH et al. piRNA-guided co-transcriptional silencing coopts nuclear export factors. Elife 8:N/A (2019). PubMed: 31219034
- Boone PG et al. A cancer rainbow mouse for visualizing the functional genomics of oncogenic clonal expansion. Nat Commun 10:5490 (2019). PubMed: 31792216
- Ritter SC & Hackel BJ Validation and Stabilization of a Prophage Lysin of Clostridium perfringens by Using Yeast Surface Display and Coevolutionary Models. Appl Environ Microbiol 85:N/A (2019). PubMed: 30850429
- Darling TL et al. Intracellular Crosslinking of Filoviral Nucleoproteins with Xintrabodies Restricts Viral Packaging. Front Immunol 8:1197 (2017). PubMed: 29021793