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    ha-tag-antibody-chip-grade-ab9110.pdf

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Tags & Cell Markers Epitope Tags HA Tag
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Anti-HA tag antibody - ChIP Grade (ab9110)

  • Datasheet
  • SDS
Reviews (56)Q&A (12)References (743)

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Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody - ChIP Grade (ab9110)
  • Immunoprecipitation - Anti-HA tag antibody - ChIP Grade (ab9110)
  • Western blot - Anti-HA tag antibody - ChIP Grade (ab9110)
  • ChIP - Anti-HA tag antibody - ChIP Grade (ab9110)
  • ChIP - Anti-HA tag antibody - ChIP Grade (ab9110)
  • Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody - ChIP Grade (ab9110)
  • Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody - ChIP Grade (ab9110)
  • Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody - ChIP Grade (ab9110)

Key features and details

  • Rabbit polyclonal to HA tag - ChIP Grade
  • Suitable for: ChIP/Chip, IP, ELISA, WB, ICC/IF, ICC, Flow Cyt, ChIP
  • Reacts with: Species independent
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-HA tag antibody [EPR22819-101] (ab236632)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-HA tag antibody - ChIP Grade
    See all HA tag primary antibodies
  • Description

    Rabbit polyclonal to HA tag - ChIP Grade
  • Host species

    Rabbit
  • Specificity

    ELISA: The anti HA diluted 1:70.000 gave an O.D.=1.0 in a 15 minute reaction against peptide conjugated with a different carrier than used for anti peptide purification. HRP conjugated Goat anti rabbit IgG was used and TMB was the substrate.
  • Tested applications

    Suitable for: ChIP/Chip, IP, ELISA, WB, ICC/IF, ICC, Flow Cyt, ChIPmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    YPYDVPDYA (influenza hemagglutinin-HA-epitope) conjugated to KLH.

  • Positive control

    • WB: 293FT cells transfected with 15kDa HA tagged Vpr (an HIV1 accessory protein). IP: Nuclear lysate of HEK-293T cells transiently expressing HA-tagged protein. ICC/IF: U-2 cells. Mouse olineu cells. ChIP: Xenopus laevis oocytes were injected with mRNA for HA-tagged human BORIS.
  • General notes

      

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.1% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Antibodies were immunoaffinity purified using the peptide conjugated to a solid-phase support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Epitope Tags
    • HA Tag
    • Signal Transduction
    • Antibodies
    • ha tag

Associated products

  • ChIP Related Products

    • Rabbit Anti-Mouse IgG H&L (ab46540)
    • ChIP Kit (ab500)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Related Products

    • Prestained Protein Ladder - Broad molecular weight (10-245 kDa) (ab116028)
    • HA tag peptide (ab13835)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab9110 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ChIP/Chip (1)
Use at an assay dependent concentration.
IP (12)
Use at an assay dependent concentration.
ELISA
1/200 - 1/500.
WB (29)
1/4000 - 1/10000.
ICC/IF (8)
Use a concentration of 1 - 4 µg/ml.
ICC (1)
Use at an assay dependent concentration.
Flow Cyt (1)
Use at an assay dependent concentration.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

ChIP (2)
Use 3 µg for 25 µg of chromatin.
Notes
ChIP/Chip
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
ELISA
1/200 - 1/500.
WB
1/4000 - 1/10000.
ICC/IF
Use a concentration of 1 - 4 µg/ml.
ICC
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

ChIP
Use 3 µg for 25 µg of chromatin.

Target

  • Relevance

    Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
  • Alternative names

    • HA epitope tag antibody
    • HA1 antibody
    • HA2 antibody
    • hemagglutinin antibody
    • Hemagglutinin HA1 chain antibody
    • Hemagglutinin HA2 chain antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody - ChIP Grade (ab9110)
    Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody - ChIP Grade (ab9110)This image is courtesy of an Abreview

    ab9110 staining HA-tagged proteins in HeLa cells by ICC/IF (Immunocytochemistry/immunoflurescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% saponin and blocked with 3% serum for 30 minutes at 37°C. Samples were incubated with primary antibody (2 µg/ml) in 1x PBS for 1 hour at 37°C. An Alexa Fluor® 488-conjugated Goat polyclonal to rabbit was used as secondary antibody. 

    See Abreview

  • Immunoprecipitation - Anti-HA tag antibody - ChIP Grade (ab9110)
    Immunoprecipitation - Anti-HA tag antibody - ChIP Grade (ab9110)This image is courtesy of an anonymous Abreview

    ab9110 was diluted to 4 µg/mg lysate and incubated with a nuclear lysate of HEK293T cells transiently expressing HA-tagged protein and a Protein A matrix for 2 hours a 23°C to achieve immunoprecipitation.  1000 µg of lysate was present in the input. 

    A HRP-conjugated anti-rabbit HA monoclonal antibody diluted 1/1000 was used for the Western Blot step.

    See Abreview

  • Western blot - Anti-HA tag antibody - ChIP Grade (ab9110)
    Western blot - Anti-HA tag antibody - ChIP Grade (ab9110)
    All lanes : Anti-HA tag antibody - ChIP Grade (ab9110) at 1/4000 dilution

    Lane 1 : 15ug untransfected wcl lysate
    Lane 2 : 293FT cells transfected with 15kDa HA tagged Vpr (an HIV1 accessory protein)

    Secondary
    All lanes : HRP conjugated Goat anti-Rabbit

    Developed using the ECL technique.

    Performed under reducing conditions.

    Exposure time: 5 seconds


    This image is courtesy of an Abreview

    See Abreview

  • ChIP - Anti-HA tag antibody - ChIP Grade (ab9110)
    ChIP - Anti-HA tag antibody - ChIP Grade (ab9110)

    Xenopus laevis oocytes were injected with mRNA for HA-tagged human BORIS. Chromatin was prepared according to the Abcam X-ChIP protocol. Oocytes were fixed with formaldehyde for 10min. The  ChIP was performed with 25µg of chromatin, 20µl of Protein A/G sepharose beads, and 3µg of ab9110 (anti-HA, light blue) or, 3µg of ab18337 (anti-Boris, dark blue). A non-specific antibody was used as a control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach).

  • ChIP - Anti-HA tag antibody - ChIP Grade (ab9110)
    ChIP - Anti-HA tag antibody - ChIP Grade (ab9110)This image is courtesy of an Abreview submitted by Mr. Dan Stummer

    Chromatin was prepared according to the X-ChIP protocol. Mouse embryonic stem whole cell lysate treated with disuccinimidyl glutarate (cross-linking agent). ChIP was performed using ab9110 at 1/200 dilution for 16 hours at 4°C in RIPA diluent. The bound DNA was quantitated by real-time PCR. Negative control: The parent cell line. Positive control: A cell line, which stably express an HA-tagged RARgamma protein.

     
     

    See Abreview

  • Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody - ChIP Grade (ab9110)
    Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody - ChIP Grade (ab9110)Seitz, K. et al Send to PLoS One. 2014 Jan 29;9(1):e87132. doi: 10.1371/journal.pone.0087132. eCollection 2014 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Arrb2 depends on Gβγ to induce membrane translocation of PKCα

    Xenopus embryos were injected with 500 pg pkcα-gfp RNA and co-injected as indicated above the images. Animal Caps were prepared at stage 10 and immunostained as indicated. Nuclei were stained with Hoechst 33258 (blue). Images show representative results from at least two independent experiments with a minimum of six Animal Caps per experiment. Scale bars: 50 µm.

    The inhibitory effect of Arrb2 MO1 (E) on PKCα-GFP membrane translocation was rescued by (F) co-injection of HA-Gβ and HA-Gγ mRNA (anti-HA (red): F′, merge: F").

    HA was detected with ab9110.

    (After Figure 2 of Seitz et al)

     

  • Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody - ChIP Grade (ab9110)
    Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody - ChIP Grade (ab9110)
    This image was kindly supplied as part of the review submitted by Kasper Fugger. Immunofluorescence staining of U-2 cells expressing HA-tagged protein with ab9110.
  • Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody - ChIP Grade (ab9110)
    Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody - ChIP Grade (ab9110)

    ab 9110 at a 1/200 dilution staining the mouse olineu cell line (oligodendrocyte precursor cell) by immunocytochemistry. The antibody was incubated with the cells for 30 minutes and then detected using a Cy5 conjugated goat anti-rabbit antibody.

    This image is courtesy of an Abreview submitted by Katarina Trajkovic on 15 March 2006

    See Abreview

Protocols

  • ChIP protocols
  • Flow cytometry protocols
  • Immunoprecipitation protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (743)

    Publishing research using ab9110? Please let us know so that we can cite the reference in this datasheet.

    ab9110 has been referenced in 743 publications.

    • Ishiguro KI  et al. MEIOSIN Directs the Switch from Mitosis to Meiosis in Mammalian Germ Cells. Dev Cell N/A:N/A (2020). PubMed: 32032549
    • Liu J  et al. USP12 translocation maintains interferon antiviral efficacy by inhibiting CBP acetyltransferase activity. PLoS Pathog 16:e1008215 (2020). PubMed: 31899788
    • Benhadou F  et al. Epidermal autonomous VEGFA/Flt1/Nrp1 functions mediate psoriasis-like disease. Sci Adv 6:eaax5849 (2020). PubMed: 31934626
    • Li Q  et al. p53 Integrates Temporal WDR5 Inputs during Neuroectoderm and Mesoderm Differentiation of Mouse Embryonic Stem Cells. Cell Rep 30:465-480.e6 (2020). PubMed: 31940490
    • Bi Y  et al. Decreased ZNF750 promotes angiogenesis in a paracrine manner via activating DANCR/miR-4707-3p/FOXC2 axis in esophageal squamous cell carcinoma. Cell Death Dis 11:296 (2020). PubMed: 32341351
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-10 of 12 Abreviews or Q&A

    Immunoprecipitation abreview for Anti-HA tag antibody - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunoprecipitation
    Sample
    Mouse Cell lysate - whole cell (Co-IP from mouse NSCs)
    Total protein in input
    500 µg
    Immuno-precipitation step
    Protein A/G
    Specification
    Co-IP from mouse NSCs
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 01 2018

    Immunoprecipitation abreview for Anti-HA tag antibody - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    Application
    Immunoprecipitation
    Immuno-precipitation step
    Protein G
    Sample
    Human Cell lysate - whole cell (HeLa)
    Specification
    HeLa
    Treatment
    transfected with HA-tagged gene
    Total protein in input
    10 µg
    Read More

    Abcam user community

    Verified customer

    Submitted May 02 2014

    Immunoprecipitation abreview for Anti-HA tag antibody - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    Application
    Immunoprecipitation
    Immuno-precipitation step
    Protein A/G
    Sample
    Human Cell lysate - nuclear (Prostate cancer cell lines)
    Specification
    Prostate cancer cell lines
    Treatment
    4 h DHT (or ETOH)
    Total protein in input
    5e+006 cells
    Read More

    Laura Cato

    Verified customer

    Submitted Apr 28 2014

    Immunoprecipitation abreview for Anti-HA tag antibody - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunoprecipitation
    Immuno-precipitation step
    A second antibody
    Sample
    Mouse Cell lysate - whole cell (Embryonic stem cell)
    Specification
    Embryonic stem cell
    Total protein in input
    500 µg
    Read More

    Mr. Dan Stummer

    Verified customer

    Submitted Apr 03 2014

    Immunoprecipitation abreview for Anti-HA tag antibody - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunoprecipitation
    Immuno-precipitation step
    Other - Magnetic Protein G
    Sample
    Human Cell lysate - whole cell (HeLa)
    Specification
    HeLa
    Total protein in input
    20 µg
    Read More

    Abcam user community

    Verified customer

    Submitted Jul 02 2013

    Immunoprecipitation abreview for Anti-HA tag antibody - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunoprecipitation
    Sample
    Human Cell lysate - whole cell (HA-tagged DNA expressing 293T cells)
    Total protein in input
    500 µg
    Specification
    HA-tagged DNA expressing 293T cells
    Immuno-precipitation step
    Protein A
    Read More

    Mr. Sungsin Jo

    Verified customer

    Submitted Apr 02 2013

    Immunoprecipitation abreview for Anti-HA tag antibody - ChIP Grade

    Good
    Abreviews
    Abreviews
    Application
    Immunoprecipitation
    Sample
    Human Cell lysate - whole cell (HEK293)
    Total protein in input
    100 µg
    Specification
    HEK293
    Treatment
    transfected with HA tagged protein
    Immuno-precipitation step
    Protein A
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 15 2012

    Immunoprecipitation abreview for Anti-HA tag antibody - ChIP Grade

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunoprecipitation
    Sample
    Human Cell lysate - whole cell (CCL13)
    Total protein in input
    100 µg
    Specification
    CCL13
    Immuno-precipitation step
    Protein A
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 10 2008

    Immunoprecipitation abreview for Anti-HA tag antibody - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunoprecipitation
    Sample
    Human Cell lysate - nuclear (HEK293T transiently expressing HA-tagged protein)
    Total protein in input
    1000 µg
    Specification
    HEK293T transiently expressing HA-tagged protein
    Immuno-precipitation step
    Protein A
    Read More

    Abcam user community

    Verified customer

    Submitted Apr 11 2008

    Immunoprecipitation abreview for Anti-HA tag antibody - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunoprecipitation
    Sample
    Human Cell lysate - whole cell (HeLa Cells transfected with HA-Spartin)
    Total protein in input
    8 µg
    Specification
    HeLa Cells transfected with HA-Spartin
    Immuno-precipitation step
    Protein A
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 04 2008

    1-10 of 12 Abreviews or Q&A

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