Immunoprecipitation abreview for Anti-HA tag antibody - ChIP Grade

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Abreviews
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Application
Immunoprecipitation
Sample
Human Cell lysate - nuclear (HEK293T transiently expressing HA-tagged protein)
Total protein in input
1000 µg
Specification
HEK293T transiently expressing HA-tagged protein
Immuno-precipitation step
Protein A

Other product details

Concentration
4 µg/mg lysate
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 23°C · Diluent: HEPES pH7.4, 140 mM KCl, 2 mM EDTA

Western Blot antibody

Name
Non-Abcam antibody was used: anti HA monoclonal Ab from Sigma
Host species: Mouse
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/1000

Additional data

Additional Notes
This Ab pulled down about 50% of HA-tagged protein from nuclear lysate of HEK293T after transient overexpression. A few nonspecific bands were observed (lane 2). Nevertheless, it was very good antibody for CO-IP experiment.

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Submitted Apr 11 2008

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