Abcam guarantees this product to work in the species/application used in this Abreview.
Human Cell lysate - nuclear (HEK293T transiently expressing HA-tagged protein)
Total protein in input
HEK293T transiently expressing HA-tagged protein
Other product details
4 µg/mg lysate
2 hour(s) and 0 minute(s) · Temperature: 23°C · Diluent: HEPES pH7.4, 140 mM KCl, 2 mM EDTA
Western Blot antibody
Non-Abcam antibody was used: anti HA monoclonal Ab from Sigma Host species: Mouse Clonality: Monoclonal Conjugation: Horse Radish Peroxidase
This Ab pulled down about 50% of HA-tagged protein from nuclear lysate of HEK293T after transient overexpression. A few nonspecific bands were observed (lane 2). Nevertheless, it was very good antibody for CO-IP experiment.
Abcam user community
Submitted Apr 11 2008
Get resources and offers direct to your inboxSign up