RecombinantRabMAb

Recombinant Anti-HA tag antibody [EPR22819-101] (ab236632)

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Western blot - Anti-HA tag antibody [EPR22819-101] (ab236632)
  • Immunoprecipitation - Anti-HA tag antibody [EPR22819-101] (ab236632)
  • Flow Cytometry - Anti-HA tag antibody [EPR22819-101] (ab236632)
  • Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody [EPR22819-101] (ab236632)
  • Anti-HA tag antibody [EPR22819-101] (ab236632)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22819-101] to HA tag
  • Suitable for: Flow Cyt, WB, IP, ICC/IF
  • Reacts with: Species independent

Overview

  • Product name

    Anti-HA tag antibody [EPR22819-101]
    See all HA tag primary antibodies

  • Description

    Rabbit monoclonal [EPR22819-101] to HA tag

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, WB, IP, ICC/IFmore details
    Unsuitable for: ChIP

  • Species reactivity

    Reacts with: Species independent

  • Immunogen

    Synthetic peptide within HA tag aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: P03437

  • Positive control

    • WB: HEK-293T and HEK-293T transfected with TIM 4 lysates. ICC/IF: HEK-293T cells transfected with HA-tagged TATA-box-binding protein (WT) expression vector.Flow Cyt: HEK-293T transfected with TATA-box-binding protein (WT) expression vector containing HA-tag cells. IP: HEK-293T transfected with TIM 4 (WT) expression vector containing a HA-GFP-tag and PDGFR TM domain whole cell lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR22819-101

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab236632 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/600.
WB 1/1000.
IP 1/30.
ICC/IF 1/1000.
  • Application notes
    Is unsuitable for ChIP.

    • Target

    • Relevance

      Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.

    • Alternative names

      • HA epitope tag antibody
      • HA1 antibody
      • HA2 antibody
      • hemagglutinin antibody
      • Hemagglutinin HA1 chain antibody
      • Hemagglutinin HA2 chain antibody
      see all

    Images

    • Western blot - Anti-HA tag antibody [EPR22819-101] (ab236632)
      Western blot - Anti-HA tag antibody [EPR22819-101] (ab236632)
      All lanes : Anti-HA tag antibody [EPR22819-101] (ab236632) at 1/1000 dilution

      Lane 1 : HEK-293T (human embryonic kidney) transfected with an empty vector (vector control), containing a HA-myc-His-GFP-tag and PDGFR TM domain, whole cell lysate
      Lane 2 : HEK-293T transfected with TIM 4 (WT) expression vector containing a HA-myc-His-GFP-tag and PDGFR TM domain, whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Observed band size: 37,78 kDa
      why is the actual band size different from the predicted?



      Antibodies used in the control panels are (Anti-TIM 4 antibody), ab9110 (Anti-HA tag antibody - ChIP Grade) and ab181602 (Anti-GAPDH antibody [EPR16891] - Loading Control).

      Exposure time: 3 minutes.

      Blocking and Diluting Buffer and concentration: 5% NFDM/TBST.

    • Immunoprecipitation - Anti-HA tag antibody [EPR22819-101] (ab236632)
      Immunoprecipitation - Anti-HA tag antibody [EPR22819-101] (ab236632)

      HA tag was immunoprecipitated from 0.35 mg HEK-293T (human embryonic kidney) transfected with TIM 4 (WT) expression vector containing a HA-GFP-tag and PDGFR TM domain whole cell lysate 10µg with ab236632 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab236632. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/2000 dilution.

      Lane 1: HEK-293T (human embryonic kidney) transfected with TIM 4 (WT) expression vector containing a HA-GFP-tag and PDGFR TM domain whole cell lysate 10µg.

      Lane 2: ab236632 IP in HEK-293T transfected with TIM 4 (WT) expression vector containing a HA-GFP-tag and PDGFR TM domain whole cell lysate.

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab236632 in HEK-293T transfected with TIM 4 (WT) expression vector containing a HA-GFP-tag and PDGFR TM domain whole cell lysate.

      Blocking and dilution buffer and concentration/ 5% NFDM/TBST.

      Exposure time: 3 seconds.

    • Flow Cytometry - Anti-HA tag antibody [EPR22819-101] (ab236632)
      Flow Cytometry - Anti-HA tag antibody [EPR22819-101] (ab236632)

      Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized HEK-293T (human embryonic kidney) transfected with TATA-box-binding protein (WT) expression vector containing HA-tag (Right) / HEK-293T (Left) cells labeling HA tag with ab236632 at 1/600 dilution. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    • Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody [EPR22819-101] (ab236632)
      Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody [EPR22819-101] (ab236632)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T (human embryonic kidney epithelial cell) transfected with HA-tagged TATA-box-binding protein (WT) expression vector labeling HA tag with ab236632 at 1/1000 (0.6 µg/ml) dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000, (2 µg/ml) dilution (Green). Confocal image showing nuclear staining in HEK-293T cells transfected with HA-tagged TATA-box-binding protein (WT) expression vector is observed. Anti-HA.11 Epitope Tag antibody (Alexa Fluor® 647) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 µg/ml) dilution.

    • Anti-HA tag antibody [EPR22819-101] (ab236632)
      Anti-HA tag antibody [EPR22819-101] (ab236632)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab236632? Please let us know so that we can cite the reference in this datasheet.

    ab236632 has not yet been referenced specifically in any publications.

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