Recombinant Anti-HA tag antibody [EPR22819-101] (ab236632)
![Western blot - Anti-HA tag antibody [EPR22819-101] (ab236632)](https://www.abcam.com/ps/products/236/ab236632/Images/ab236632-353131-anti-ha-tag-epr22819-101-western-blot-human.jpg "Western blot - Anti-HA tag antibody [EPR22819-101] (ab236632)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22819-101] to HA tag
- Suitable for: Flow Cyt (Intra), WB, IP, ICC/IF
- Reacts with: Species independent
Related conjugates and formulations
Overview
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Product name
Anti-HA tag antibody [EPR22819-101]
See all HA tag primary antibodies -
Description
Rabbit monoclonal [EPR22819-101] to HA tag -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IP, ICC/IFmore details
Unsuitable for: ChIP -
Species reactivity
Reacts with: Species independent -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HEK-293T and HEK-293T transfected with TIM 4 lysates. ICC/IF: HEK-293T cells transfected with HA-tagged TATA-box-binding protein (WT) expression vector.Flow Cyt (intra): HEK-293T transfected with TATA-box-binding protein (WT) expression vector containing HA-tag cells. IP: HEK-293T transfected with TIM 4 (WT) expression vector containing a HA-GFP-tag and PDGFR TM domain whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22819-101 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab236632 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/600.
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| WB |
1/1000.
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| IP |
1/30.
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| ICC/IF |
1/1000.
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| Notes |
|---|
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Flow Cyt (Intra)
1/600. |
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WB
1/1000. |
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IP
1/30. |
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ICC/IF
1/1000. |
Target
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Relevance
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins. -
Alternative names
- HA epitope tag antibody
- HA1 antibody
- HA2 antibody
see all
Images
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Western blot - Anti-HA tag antibody [EPR22819-101] (ab236632)All lanes : Anti-HA tag antibody [EPR22819-101] (ab236632) at 1/1000 dilution
Lane 1 : HEK-293T (human embryonic kidney) transfected with an empty vector (vector control), containing a HA-myc-His-GFP-tag and PDGFR TM domain, whole cell lysate
Lane 2 : HEK-293T transfected with TIM 4 (WT) expression vector containing a HA-myc-His-GFP-tag and PDGFR TM domain, whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Observed band size: 37,78 kDa why is the actual band size different from the predicted?Antibodies used in the control panels are (Anti-TIM 4 antibody), ab9110 (Anti-HA tag antibody - ChIP Grade) and ab181602 (Anti-GAPDH antibody [EPR16891] - Loading Control).
Exposure time: 3 minutes.
Blocking and Diluting Buffer and concentration: 5% NFDM/TBST.
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![Immunoprecipitation - Anti-HA tag antibody [EPR22819-101] (ab236632)](https://www.abcam.com/ps/products/236/ab236632/Images/ab236632-3-anti-ha-tag-epr22819-101-immunoprecipitation-hek293t-overexpression-human.jpg)
Immunoprecipitation - Anti-HA tag antibody [EPR22819-101] (ab236632)HA tag was immunoprecipitated from 0.35 mg HEK-293T (human embryonic kidney) transfected with TIM 4 (WT) expression vector containing a HA-GFP-tag and PDGFR TM domain whole cell lysate 10µg with ab236632 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab236632. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/2000 dilution.
Lane 1: HEK-293T (human embryonic kidney) transfected with TIM 4 (WT) expression vector containing a HA-GFP-tag and PDGFR TM domain whole cell lysate 10µg.
Lane 2: ab236632 IP in HEK-293T transfected with TIM 4 (WT) expression vector containing a HA-GFP-tag and PDGFR TM domain whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab236632 in HEK-293T transfected with TIM 4 (WT) expression vector containing a HA-GFP-tag and PDGFR TM domain whole cell lysate.
Blocking and dilution buffer and concentration/ 5% NFDM/TBST.
Exposure time: 3 seconds.
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![Flow Cytometry (Intracellular) - Anti-HA tag antibody [EPR22819-101] (ab236632)](https://www.abcam.com/ps/products/236/ab236632/Images/ab236632-2-anti-ha-tag-epr22819-101-flowcytometry-hek293t-overexpression-human.jpg)
Flow Cytometry (Intracellular) - Anti-HA tag antibody [EPR22819-101] (ab236632)Intracellular flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized HEK-293T (human embryonic kidney) transfected with TATA-box-binding protein (WT) expression vector containing HA-tag (Right) / HEK-293T (Left) cells labeling HA tag with ab236632 at 1/600 dilution. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
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![Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody [EPR22819-101] (ab236632)](https://www.abcam.com/ps/products/236/ab236632/Images/ab236632-1-anti-ha-tag-epr22819-101-immunocytochemistry-293T-human.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody [EPR22819-101] (ab236632)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T (human embryonic kidney epithelial cell) transfected with HA-tagged TATA-box-binding protein (WT) expression vector labeling HA tag with ab236632 at 1/1000 (0.6 µg/ml) dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000, (2 µg/ml) dilution (Green). Confocal image showing nuclear staining in HEK-293T cells transfected with HA-tagged TATA-box-binding protein (WT) expression vector is observed. Anti-HA.11 Epitope Tag antibody (Alexa Fluor® 647) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 µg/ml) dilution.
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![Anti-HA tag antibody [EPR22819-101] (ab236632)](https://www.abcam.com/ps/products/236/ab236632/Images/ab236632-5-benefits-of-recombinant-antibodies.png)
Anti-HA tag antibody [EPR22819-101] (ab236632)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (6)
ab236632 has been referenced in 6 publications.
- Wang B et al. circNRIP1 facilitates keloid progression via FXR1-mediated upregulation of miR-503-3p and miR-503-5p. Int J Mol Med 47:N/A (2021). PubMed: 33649815
- Hu Z et al. Discovery and engineering of small SlugCas9 with broad targeting range and high specificity and activity. Nucleic Acids Res 49:4008-4019 (2021). PubMed: 33721016
- Dhaouadi S et al. Novel Human Tenascin-C Function-Blocking Camel Single Domain Nanobodies. Front Immunol 12:635166 (2021). PubMed: 33790905
- Cheng K et al. Bioengineered bacteria-derived outer membrane vesicles as a versatile antigen display platform for tumor vaccination via Plug-and-Display technology. Nat Commun 12:2041 (2021). PubMed: 33824314
- Xu Y et al. MiRNA-340-5p mediates the functional and infiltrative promotion of tumor-infiltrating CD8+ T lymphocytes in human diffuse large B cell lymphoma. J Exp Clin Cancer Res 39:238 (2020). PubMed: 33168024
- Zhuang W & Yan Z The S2-S3 Loop of Kv7.4 Channels Is Essential for Calmodulin Regulation of Channel Activation. Front Physiol 11:604134 (2020). PubMed: 33551832