Recombinant
RabMAb

Recombinant Anti-HA tag antibody [EPR22819-101] (ab236632)

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Overview

  • Product name

    Anti-HA tag antibody [EPR22819-101]
    See all HA tag primary antibodies

  • Description

    Rabbit monoclonal [EPR22819-101] to HA tag

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, WB, IP, ICC/IFmore details
    Unsuitable for: ChIP

  • Species reactivity

    Reacts with: Species independent

  • Immunogen

    Synthetic peptide within HA tag aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: P03437

  • Positive control

    • WB: HEK-293T and HEK-293T transfected with TIM 4 lysates. ICC/IF: HEK-293T cells transfected with HA-tagged TATA-box-binding protein (WT) expression vector. FC: HEK-293T transfected with TATA-box-binding protein (WT) expression vector containing HA-tag cells. IP: HEK-293T transfected with TIM 4 (WT) expression vector containing a HA-GFP-tag and PDGFR TM domain whole cell lysate.

  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR22819-101

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab236632 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/600.
WB 1/1000.
IP 1/30.
ICC/IF 1/1000.
  • Application notes
    Is unsuitable for ChIP.

    • Target

    • Relevance

      Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.

    • Alternative names

      • HA epitope tag antibody
      • HA1 antibody
      • HA2 antibody
      • hemagglutinin antibody
      • Hemagglutinin HA1 chain antibody
      • Hemagglutinin HA2 chain antibody
      see all

    Images

    • Western blot - Anti-HA tag antibody [EPR22819-101] (ab236632)
      Western blot - Anti-HA tag antibody [EPR22819-101] (ab236632)
      All lanes : Anti-HA tag antibody [EPR22819-101] (ab236632) at 1/1000 dilution

      Lane 1 : HEK-293T (human embryonic kidney) transfected with an empty vector (vector control), containing a HA-myc-His-GFP-tag and PDGFR TM domain, whole cell lysate
      Lane 2 : HEK-293T transfected with TIM 4 (WT) expression vector containing a HA-myc-His-GFP-tag and PDGFR TM domain, whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Observed band size: 37,78 kDa
      why is the actual band size different from the predicted?



      Antibodies used in the control panels are (Anti-TIM 4 antibody), ab9110 (Anti-HA tag antibody - ChIP Grade) and ab181602 (Anti-GAPDH antibody [EPR16891] - Loading Control).

      Exposure time: 3 minutes.

      Blocking and Diluting Buffer and concentration: 5% NFDM/TBST.

    • Immunoprecipitation - Anti-HA tag antibody [EPR22819-101] (ab236632)
      Immunoprecipitation - Anti-HA tag antibody [EPR22819-101] (ab236632)

      HA tag was immunoprecipitated from 0.35 mg HEK-293T (human embryonic kidney) transfected with TIM 4 (WT) expression vector containing a HA-GFP-tag and PDGFR TM domain whole cell lysate 10µg with ab236632 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab236632. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/2000 dilution.

      Lane 1: HEK-293T (human embryonic kidney) transfected with TIM 4 (WT) expression vector containing a HA-GFP-tag and PDGFR TM domain whole cell lysate 10µg.

      Lane 2: ab236632 IP in HEK-293T transfected with TIM 4 (WT) expression vector containing a HA-GFP-tag and PDGFR TM domain whole cell lysate.

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab236632 in HEK-293T transfected with TIM 4 (WT) expression vector containing a HA-GFP-tag and PDGFR TM domain whole cell lysate.

      Blocking and dilution buffer and concentration/ 5% NFDM/TBST.

      Exposure time: 3 seconds.

    • Flow Cytometry - Anti-HA tag antibody [EPR22819-101] (ab236632)
      Flow Cytometry - Anti-HA tag antibody [EPR22819-101] (ab236632)

      Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized HEK-293T (human embryonic kidney) transfected with TATA-box-binding protein (WT) expression vector containing HA-tag (Right) / HEK-293T (Left) cells labeling HA tag with ab236632 at 1/600 dilution. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    • Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody [EPR22819-101] (ab236632)
      Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody [EPR22819-101] (ab236632)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T (human embryonic kidney epithelial cell) transfected with HA-tagged TATA-box-binding protein (WT) expression vector labeling HA tag with ab236632 at 1/1000 (0.6 µg/ml) dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000, (2 µg/ml) dilution (Green). Confocal image showing nuclear staining in HEK-293T cells transfected with HA-tagged TATA-box-binding protein (WT) expression vector is observed. Anti-HA.11 Epitope Tag antibody (Alexa Fluor® 647) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 µg/ml) dilution.

    References

    ab236632 has not yet been referenced specifically in any publications.

    Publishing research using ab236632? Please let us know so that we can cite the reference in this datasheet.

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