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RecombinantRabMAb

Recombinant Anti-HA tag antibody [EPR22819-101] - BSA and Azide free (ab256483)

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Immunoprecipitation - Anti-HA tag antibody [EPR22819-101] - BSA and Azide free (ab256483)
  • Flow Cytometry (Intracellular) - Anti-HA tag antibody [EPR22819-101] - BSA and Azide free (ab256483)
  • Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody [EPR22819-101] - BSA and Azide free (ab256483)
  • Anti-HA tag antibody [EPR22819-101] - BSA and Azide free (ab256483)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22819-101] to HA tag - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), WB, IP, ICC/IF
  • Reacts with: Species independent

Conjugates logo Related conjugates and formulations

Alexa Fluor® 647 Unconjugated

Overview

  • Product name

    Anti-HA tag antibody [EPR22819-101] - BSA and Azide free
    See all HA tag primary antibodies

  • Description

    Rabbit monoclonal [EPR22819-101] to HA tag - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, IP, ICC/IFmore details
    Unsuitable for: ChIP

  • Species reactivity

    Reacts with: Species independent

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK-293T and HEK-293T transfected with TIM 4 lysates. ICC/IF: HEK-293T cells transfected with HA-tagged TATA-box-binding protein (WT) expression vector.Flow Cyt (intra): HEK-293T transfected with TATA-box-binding protein (WT) expression vector containing HA-tag cells. IP: HEK-293T transfected with TIM 4 (WT) expression vector containing a HA-GFP-tag and PDGFR TM domain whole cell lysate.

  • General notes

    ab256483 is the carrier-free version of ab236632.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR22819-101

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab256483 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
Notes
Flow Cyt (Intra)
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
Application notes
Is unsuitable for ChIP.

Target

  • Relevance

    Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.

  • Alternative names

    • HA epitope tag antibody
    • HA1 antibody
    • HA2 antibody
    • hemagglutinin antibody
    • Hemagglutinin HA1 chain antibody
    • Hemagglutinin HA2 chain antibody
    see all

Images

  • Immunoprecipitation - Anti-HA tag antibody [EPR22819-101] - BSA and Azide free (ab256483)
    Immunoprecipitation - Anti-HA tag antibody [EPR22819-101] - BSA and Azide free (ab256483)

    HA tag was immunoprecipitated from 0.35 mg HEK-293T (human embryonic kidney) transfected with TIM 4 (WT) expression vector containing a HA-GFP-tag and PDGFR TM domain whole cell lysate 10µg with ab236632 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab236632. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/2000 dilution.

    Lane 1: HEK-293T (human embryonic kidney) transfected with TIM 4 (WT) expression vector containing a HA-GFP-tag and PDGFR TM domain whole cell lysate 10µg.

    Lane 2: ab236632 IP in HEK-293T transfected with TIM 4 (WT) expression vector containing a HA-GFP-tag and PDGFR TM domain whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab236632 in HEK-293T transfected with TIM 4 (WT) expression vector containing a HA-GFP-tag and PDGFR TM domain whole cell lysate.

    Blocking and dilution buffer and concentration/ 5% NFDM/TBST.

    Exposure time: 3 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab236632).

  • Flow Cytometry (Intracellular) - Anti-HA tag antibody [EPR22819-101] - BSA and Azide free (ab256483)
    Flow Cytometry (Intracellular) - Anti-HA tag antibody [EPR22819-101] - BSA and Azide free (ab256483)

    Intracellular flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized HEK-293T (human embryonic kidney) transfected with TATA-box-binding protein (WT) expression vector containing HA-tag (Right) / HEK-293T (Left) cells labeling HA tag with ab236632 at 1/600 dilution. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. 

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab236632). 

     

     

  • Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody [EPR22819-101] - BSA and Azide free (ab256483)
    Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody [EPR22819-101] - BSA and Azide free (ab256483)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T (human embryonic kidney epithelial cell) transfected with HA-tagged TATA-box-binding protein (WT) expression vector labeling HA tag with ab236632 at 1/1000 (0.6 µg/ml) dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000, 2 µg/ml dilution (Green). Confocal image showing nuclear staining in HEK-293T cells transfected with HA-tagged TATA-box-binding protein (WT) expression vector is observed. Anti-HA.11 Epitope Tag antibody (Alexa Fluor® 647) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 µg/ml) dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab236632).

  • Anti-HA tag antibody [EPR22819-101] - BSA and Azide free (ab256483)
    Anti-HA tag antibody [EPR22819-101] - BSA and Azide free (ab256483)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab256483? Please let us know so that we can cite the reference in this datasheet.

ab256483 has not yet been referenced specifically in any publications.

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