Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4095] to HA tag
- Suitable for: WB
- Reacts with: Species independent
Product nameAnti-HA tag antibody [EPR4095]
See all HA tag primary antibodies
DescriptionRabbit monoclonal [EPR4095] to HA tag
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Species independent
Synthetic peptide within HA tag. The exact sequence is proprietary.
- Multi-tag fusion protein. HA-tagged Human Glutaminase C overexpression lysates.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab182009 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000.|
RelevanceHuman influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
- HA epitope tag antibody
- HA1 antibody
- HA2 antibody
Anti-HA tag antibody [EPR4095] (ab182009) at 1/10000 dilution (Purified) + HA-tagged Human Glutaminase C overexpression lysates at 15 µg
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Observed band size: 57 kDa why is the actual band size different from the predicted?
Anti-HA tag antibody [EPR4095] (ab182009) at 1/5000 dilution + multi-tag fusion protein, 1 ng
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Blocking/ Dilution buffer: 5% NFDM /TBST.
ab182009 has been referenced in 1 publication.
- Driesbaugh KH et al. Proteolytic activation of the protease-activated receptor (PAR)-2 by the glycosylphosphatidylinositol-anchored serine protease testisin. J Biol Chem 290:3529-41 (2015). Human . PubMed: 25519908