Overview

  • Product name
    Anti-HA tag antibody (FITC)
    See all HA tag primary antibodies
  • Description
    Rabbit polyclonal to HA tag (FITC)
  • Host species
    Rabbit
  • Conjugation
    FITC. Ex: 493nm, Em: 528nm
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Immunogen

    YPYDVPDYA (influenza hemagglutinin-HA-epitope) conjugated to KLH.

  • General notes

    Molar F/P ratio is 3.7:1.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C. Store In the Dark.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
    Constituents: 0.2% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Antibodies were immunoaffinity purified using the peptide conjugated to a solid-phase support and conjugated to fluorescein isothiocyanate.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab1208 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution. Predicted molecular weight: 1 kDa.
ICC/IF Use at an assay dependent concentration.

Target

  • Relevance
    Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
  • Alternative names
    • HA epitope tag antibody
    • HA1 antibody
    • HA2 antibody
    • hemagglutinin antibody
    • Hemagglutinin HA1 chain antibody
    • Hemagglutinin HA2 chain antibody
    see all

Images

  • ab1208 staining Ha tag in human breast cancer cells (HA tagged expression plasmid) by Immunocytochemistry/ Immunofluorescence. The cells were paraformaldehyde fixed, permeabilised in 0.2% Triton X-100 in PBS and then blocked using 1% BSA and 3% FBS in PBS for 30 minutes at room temperature. Samples were then incubated with primary antibody at 1/50 for 1 hour.

    See Abreview

References

This product has been referenced in:
  • Polpitiya Arachchige S  et al. Analysis of Select Herpes Simplex Virus 1 (HSV-1) Proteins for Restriction of Human Immunodeficiency Virus Type 1 (HIV-1): HSV-1 gM Protein Potently Restricts HIV-1 by Preventing Intracellular Transport and Processing of Env gp160. J Virol 92:N/A (2018). Read more (PubMed: 29093081) »
  • Decker CE  et al. Tmem178 acts in a novel negative feedback loop targeting NFATc1 to regulate bone mass. Proc Natl Acad Sci U S A 112:15654-9 (2015). Read more (PubMed: 26644563) »
See all 2 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Monkey Cell (cos7)
Specification
cos7
Permeabilization
No
Fixative
Formaldehyde

Ms. Ya Zhou

Verified customer

Submitted Dec 13 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Breast cancer cells (HA tagged expression plasmid)
Specification
Breast cancer cells (HA tagged expression plasmid
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.2% Triton in PBS
Blocking step
1% BSA and 3% FBS in PBS as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C

Dr. Manikandan Periyasamy

Verified customer

Submitted Sep 23 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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