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  1. Link

    ha-tag-antibody-ha-7-ab49969.pdf

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Tags & Cell Markers Epitope Tags HA Tag
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Anti-HA tag antibody [HA-7] (ab49969)

  • Datasheet
  • SDS
Reviews (5) Submit a question References (10)

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Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody [HA-7] (ab49969)
  • Immunoprecipitation - Anti-HA tag antibody [HA-7] (ab49969)
  • Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody [HA-7] (ab49969)

Key features and details

  • Mouse monoclonal [HA-7] to HA tag
  • Suitable for: ELISA, ICC/IF, IP, WB
  • Reacts with: Species independent
  • Isotype: IgG1

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Overview

  • Product name

    Anti-HA tag antibody [HA-7]
    See all HA tag primary antibodies
  • Description

    Mouse monoclonal [HA-7] to HA tag
  • Host species

    Mouse
  • Tested applications

    Suitable for: ELISA, ICC/IF, IP, WBmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Synthetic peptide:

    YPYDVPDYA

    conjugated to KLH, corresponding to amino acids 98-106 of Influenza A Virus Hemagglutinin HA tag
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • Whole extracts of transfected 293T (human embryonal kidney) cells, expressing HA-tagged fusion protein.
  • General notes

    This product was changed from ascites to tissue culture supernatant on 25th October 2016. The following lot(s) is/are from ascites and is still in stock as of 25th October 2016 - GR275412, GR177296. Lot numbers other than GR275412 and GR177296 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.097% Sodium azide
    Constituents: 1% BSA, PBS
  • Concentration information loading...
  • Purity

    Proprietary Purification
  • Purification notes

    Purified from Tissue culture supernatant”.
  • Clonality

    Monoclonal
  • Clone number

    HA-7
  • Isotype

    IgG1
  • Research areas

    • Tags & Cell Markers
    • Epitope Tags
    • HA Tag

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab49969 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
Use at an assay dependent concentration.

Use at an assay dependent concentration.

ICC/IF
Use a concentration of 1 - 2 µg/ml.
IP (1)
Use a concentration of 0.5 - 2 µg/ml.
WB (2)
Use a concentration of 0.25 - 1 µg/ml. Predicted molecular weight: 63 kDa.
Notes
ELISA
Use at an assay dependent concentration.

Use at an assay dependent concentration.

ICC/IF
Use a concentration of 1 - 2 µg/ml.
IP
Use a concentration of 0.5 - 2 µg/ml.
WB
Use a concentration of 0.25 - 1 µg/ml. Predicted molecular weight: 63 kDa.

Target

  • Relevance

    Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
  • Alternative names

    • HA epitope tag antibody
    • HA1 antibody
    • HA2 antibody
    • hemagglutinin antibody
    • Hemagglutinin HA1 chain antibody
    • Hemagglutinin HA2 chain antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody [HA-7] (ab49969)
    Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody [HA-7] (ab49969)

    HEK-293 cells overexpressing N-terminal HA tagged fusion protein were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton™ X-100. Fixed cells were stained with 1 μg/mL Anti-HA tag antibody [HA-7] (ab49969). The antibody was developed using Goat Anti-Mouse IgG, Cy3™ conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.

  • Immunoprecipitation - Anti-HA tag antibody [HA-7] (ab49969)
    Immunoprecipitation - Anti-HA tag antibody [HA-7] (ab49969)This image is courtesy of an anonymous Abreview

    ab49969 Immunoprecipitating HA tag in HA tagged protein transfected 293T whole cell lysate. 25µg of cell lysate was incubated with primary antibody (1/2500 in lysis buffer) and matrix (Protein A/G) for 6 hours at 4°C. For western blotting anti-mouse HRP (ab6789) (1/10000) was used to confirm successful immunoprecipation.

    75kD is specific band for HA tagged protein. 120kD, 55kD, 35kD is non specific band for protein A/G bead.

    See Abreview

  • Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody [HA-7] (ab49969)
    Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody [HA-7] (ab49969)

    HEK-293 cells overexpressing C-terminal HA tagged fusion protein were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton™ X-100. Fixed cells were stained with 1 μg/mL Anti-HA tag antibody [HA-7] (ab49969). The antibody was developed using Goat Anti-Mouse IgG, Cy3™ conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.

Protocols

  • Immunoprecipitation protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (10)

Publishing research using ab49969? Please let us know so that we can cite the reference in this datasheet.

ab49969 has been referenced in 10 publications.

  • Shi YF  et al. Interaction between CD9 and PI3K-p85 activates the PI3K/AKT signaling pathway in B-lineage acute lymphoblastic leukemia. Oncol Rep 46:N/A (2021). PubMed: 34036396
  • Harris IS  et al. Deubiquitinases Maintain Protein Homeostasis and Survival of Cancer Cells upon Glutathione Depletion. Cell Metab 29:1166-1181.e6 (2019). PubMed: 30799286
  • Boassa D  et al. Split-miniSOG for Spatially Detecting Intracellular Protein-Protein Interactions by Correlated Light and Electron Microscopy. Cell Chem Biol 26:1407-1416.e5 (2019). PubMed: 31378710
  • Rangel LT  et al. Identification and characterization of putative Aeromonas spp. T3SS effectors. PLoS One 14:e0214035 (2019). PubMed: 31163020
  • Kunii A  et al. Three-Component Repurposed Technology for Enhanced Expression: Highly Accumulable Transcriptional Activators via Branched Tag Arrays. CRISPR J 1:337-347 (2018). PubMed: 31021277
  • Kim MY  et al. Mbd2-CP2c loop drives adult-type globin gene expression and definitive erythropoiesis. Nucleic Acids Res 46:4933-4949 (2018). PubMed: 29547954
  • Shimizu A  et al. Onset of deaminase APOBEC3B induction in response to DNA double-strand breaks. Biochem Biophys Rep 16:115-121 (2018). PubMed: 30417129
  • Guo H  et al. The aspirin-induced long non-coding RNA OLA1P2 blocks phosphorylated STAT3 homodimer formation. Genome Biol 17:24 (2016). WB ; Human . PubMed: 26898989
  • Müller AC  et al. Identifying Kinase Substrates via a Heavy ATP Kinase Assay and Quantitative Mass Spectrometry. Sci Rep 6:28107 (2016). PubMed: 27346722
  • Miotto B & Struhl K HBO1 histone acetylase activity is essential for DNA replication licensing and inhibited by Geminin. Mol Cell 37:57-66 (2010). PubMed: 20129055

Customer reviews and Q&As

Show All Reviews Q&A
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1-5 of 5 Abreviews or Q&A

Western blot abreview for Anti-HA tag antibody [HA-7]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
40 µg
Treatment
over-expression of HA/V5 double tagged protein
Specification
HEK293
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 15°C
Read More

Abcam user community

Verified customer

Submitted Jan 20 2022

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-HA tag antibody [HA-7]

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (HA-tag/Myc overexpr. MDA-MB-231 cells, inj.)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH 6.0 Citric Acid
Permeabilization
Yes - Tween-20
Specification
HA-tag/Myc overexpr. MDA-MB-231 cells, inj.
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

Submitted May 23 2017

Immunocytochemistry abreview for Anti-HA tag antibody [HA-7]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 25°C
Sample
Mouse Cell (MEL (erythroluekemia cell))
Specification
MEL (erythroluekemia cell)
Permeabilization
No
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

Submitted Jul 10 2013

Western blot abreview for Anti-HA tag antibody [HA-7]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE)
Sample
Mouse Cell lysate - whole cell (MEL (erythroluekemia cell))
Specification
MEL (erythroluekemia cell)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Read More

Abcam user community

Verified customer

Submitted Jul 09 2013

Immunoprecipitation abreview for Anti-HA tag antibody [HA-7]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunoprecipitation
Immuno-precipitation step
Protein A/G
Sample
Human Cell lysate - whole cell (293T (human kidney cell))
Specification
293T (human kidney cell)
Total protein in input
25 µg
Read More

Abcam user community

Verified customer

Submitted Jul 05 2013

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