Anti-HA tag antibody [HA-7] (ab49969)
![Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody [HA-7] (ab49969)](https://www.abcam.com/ps/products/49/ab49969/Images/ab49969-273324-anti-ha-tag-antibody-ha-7-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody [HA-7] (ab49969)")
Key features and details
- Mouse monoclonal [HA-7] to HA tag
- Suitable for: ELISA, ICC/IF, IP, WB
- Reacts with: Species independent
- Isotype: IgG1
Overview
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Product name
Anti-HA tag antibody [HA-7]
See all HA tag primary antibodies -
Description
Mouse monoclonal [HA-7] to HA tag -
Host species
Mouse -
Tested applications
Suitable for: ELISA, ICC/IF, IP, WBmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Synthetic peptide:
YPYDVPDYA
conjugated to KLH, corresponding to amino acids 98-106 of Influenza A Virus Hemagglutinin HA tag -
Positive control
- Whole extracts of transfected 293T (human embryonal kidney) cells, expressing HA-tagged fusion protein.
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General notes
This product was changed from ascites to tissue culture supernatant on 25th October 2016. The following lot(s) is/are from ascites and is still in stock as of 25th October 2016 - GR275412, GR177296. Lot numbers other than GR275412 and GR177296 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading... -
Purity
Proprietary Purification -
Purification notes
Purified from Tissue culture supernatant”. -
Clonality
Monoclonal -
Clone number
HA-7 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab49969 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
Use at an assay dependent concentration. |
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| ICC/IF |
Use a concentration of 1 - 2 µg/ml.
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| IP | (1) |
Use a concentration of 0.5 - 2 µg/ml.
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| WB | (1) |
Use a concentration of 0.25 - 1 µg/ml. Predicted molecular weight: 63 kDa.
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| Notes |
|---|
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ELISA
Use at an assay dependent concentration. Use at an assay dependent concentration. |
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ICC/IF
Use a concentration of 1 - 2 µg/ml. |
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IP
Use a concentration of 0.5 - 2 µg/ml. |
|
WB
Use a concentration of 0.25 - 1 µg/ml. Predicted molecular weight: 63 kDa. |
Target
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Relevance
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins. -
Alternative names
- HA epitope tag antibody
- HA1 antibody
- HA2 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody [HA-7] (ab49969)
HEK-293 cells overexpressing N-terminal HA tagged fusion protein were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton™ X-100. Fixed cells were stained with 1 μg/mL Anti-HA tag antibody [HA-7] (ab49969). The antibody was developed using Goat Anti-Mouse IgG, Cy3™ conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
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![Immunoprecipitation - Anti-HA tag antibody [HA-7] (ab49969)](https://www.abcam.com/ps/products/49/ab49969/Images/ab49969-198918-anti-ha-tag-antibody-ha-7-immunoprecipitation.jpg)
Immunoprecipitation - Anti-HA tag antibody [HA-7] (ab49969)This image is courtesy of an anonymous Abreviewab49969 Immunoprecipitating HA tag in HA tagged protein transfected 293T whole cell lysate. 25µg of cell lysate was incubated with primary antibody (1/2500 in lysis buffer) and matrix (Protein A/G) for 6 hours at 4°C. For western blotting anti-mouse HRP (ab6789) (1/10000) was used to confirm successful immunoprecipation.
75kD is specific band for HA tagged protein. 120kD, 55kD, 35kD is non specific band for protein A/G bead.
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![Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody [HA-7] (ab49969)](https://www.abcam.com/ps/products/49/ab49969/Images/ab49969-273325-anti-ha-tag-antibody-ha-7-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody [HA-7] (ab49969)HEK-293 cells overexpressing C-terminal HA tagged fusion protein were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton™ X-100. Fixed cells were stained with 1 μg/mL Anti-HA tag antibody [HA-7] (ab49969). The antibody was developed using Goat Anti-Mouse IgG, Cy3™ conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (9)
ab49969 has been referenced in 9 publications.
- Boassa D et al. Split-miniSOG for Spatially Detecting Intracellular Protein-Protein Interactions by Correlated Light and Electron Microscopy. Cell Chem Biol 26:1407-1416.e5 (2019). PubMed: 31378710
- Rangel LT et al. Identification and characterization of putative Aeromonas spp. T3SS effectors. PLoS One 14:e0214035 (2019). PubMed: 31163020
- Harris IS et al. Deubiquitinases Maintain Protein Homeostasis and Survival of Cancer Cells upon Glutathione Depletion. Cell Metab 29:1166-1181.e6 (2019). PubMed: 30799286
- Kunii A et al. Three-Component Repurposed Technology for Enhanced Expression: Highly Accumulable Transcriptional Activators via Branched Tag Arrays. CRISPR J 1:337-347 (2018). PubMed: 31021277
- Kim MY et al. Mbd2-CP2c loop drives adult-type globin gene expression and definitive erythropoiesis. Nucleic Acids Res 46:4933-4949 (2018). PubMed: 29547954
- Shimizu A et al. Onset of deaminase APOBEC3B induction in response to DNA double-strand breaks. Biochem Biophys Rep 16:115-121 (2018). PubMed: 30417129
- Guo H et al. The aspirin-induced long non-coding RNA OLA1P2 blocks phosphorylated STAT3 homodimer formation. Genome Biol 17:24 (2016). WB ; Human . PubMed: 26898989
- Müller AC et al. Identifying Kinase Substrates via a Heavy ATP Kinase Assay and Quantitative Mass Spectrometry. Sci Rep 6:28107 (2016). PubMed: 27346722
- Miotto B & Struhl K HBO1 histone acetylase activity is essential for DNA replication licensing and inhibited by Geminin. Mol Cell 37:57-66 (2010). PubMed: 20129055