Overview

  • Product name

    Anti-HA tag antibody [HA-7]
    See all HA tag primary antibodies
  • Description

    Mouse monoclonal [HA-7] to HA tag
  • Host species

    Mouse
  • Tested applications

    Suitable for: ELISA, ICC/IF, IP, WBmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Synthetic peptide:

    YPYDVPDYA

    conjugated to KLH, corresponding to amino acids 98-106 of Influenza A Virus Hemagglutinin HA tag

  • Positive control

    • Whole extracts of transfected 293T (human embryonal kidney) cells, expressing HA-tagged fusion protein.
  • General notes

    This product was changed from ascites to tissue culture supernatant on 25th October 2016. The following lot(s) is/are from ascites and is still in stock as of 25th October 2016 - GR275412, GR177296. Lot numbers other than GR275412 and GR177296 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.4
    Preservative: 0.097% Sodium azide
    Constituents: 1% BSA, PBS
  • Concentration information loading...
  • Purity

    Proprietary Purification
  • Purification notes

    Purified from Tissue culture supernatant”.
  • Clonality

    Monoclonal
  • Clone number

    HA-7
  • Isotype

    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab49969 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

Use at an assay dependent concentration.

ICC/IF Use a concentration of 1 - 2 µg/ml.
IP Use a concentration of 0.5 - 2 µg/ml.
WB Use a concentration of 0.25 - 1 µg/ml. Predicted molecular weight: 63 kDa.

Target

  • Relevance

    Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
  • Alternative names

    • HA epitope tag antibody
    • HA1 antibody
    • HA2 antibody
    • hemagglutinin antibody
    • Hemagglutinin HA1 chain antibody
    • Hemagglutinin HA2 chain antibody
    see all

Images

  • HEK-293 cells overexpressing N-terminal HA tagged fusion protein were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton™ X-100. Fixed cells were stained with 1 μg/mL Anti-HA tag antibody [HA-7] (ab49969). The antibody was developed using Goat Anti-Mouse IgG, Cy3™ conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.

  • ab49969 Immunoprecipitating HA tag in HA tagged protein transfected 293T whole cell lysate. 25µg of cell lysate was incubated with primary antibody (1/2500 in lysis buffer) and matrix (Protein A/G) for 6 hours at 4°C. For western blotting anti-mouse HRP (ab6789) (1/10000) was used to confirm successful immunoprecipation.

    75kD is specific band for HA tagged protein. 120kD, 55kD, 35kD is non specific band for protein A/G bead.

    See Abreview

  • HEK-293 cells overexpressing C-terminal HA tagged fusion protein were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton™ X-100. Fixed cells were stained with 1 μg/mL Anti-HA tag antibody [HA-7] (ab49969). The antibody was developed using Goat Anti-Mouse IgG, Cy3™ conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.

References

This product has been referenced in:

  • Boassa D  et al. Split-miniSOG for Spatially Detecting Intracellular Protein-Protein Interactions by Correlated Light and Electron Microscopy. Cell Chem Biol 26:1407-1416.e5 (2019). Read more (PubMed: 31378710) »
  • Kim MY  et al. Mbd2-CP2c loop drives adult-type globin gene expression and definitive erythropoiesis. Nucleic Acids Res 46:4933-4949 (2018). Read more (PubMed: 29547954) »
See all 5 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (HA-tag/Myc overexpr. MDA-MB-231 cells, inj.)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH 6.0 Citric Acid
Permeabilization
Yes - Tween-20
Specification
HA-tag/Myc overexpr. MDA-MB-231 cells, inj.
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted May 23 2017

Application
Immunocytochemistry
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 25°C
Sample
Mouse Cell (MEL (erythroluekemia cell))
Specification
MEL (erythroluekemia cell)
Permeabilization
No
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Jul 10 2013

Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE)
Sample
Mouse Cell lysate - whole cell (MEL (erythroluekemia cell))
Specification
MEL (erythroluekemia cell)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jul 09 2013

Application
Immunoprecipitation
Immuno-precipitation step
Protein A/G
Sample
Human Cell lysate - whole cell (293T (human kidney cell))
Specification
293T (human kidney cell)
Total protein in input
25 µg

Abcam user community

Verified customer

Submitted Jul 05 2013

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