Anti-HA tag antibody [HA-7] (ab49969)
- Datasheet
- References (5)
- Protocols
Overview
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Product name
Anti-HA tag antibody [HA-7]
See all HA tag primary antibodies -
Description
Mouse monoclonal [HA-7] to HA tag -
Host species
Mouse -
Tested applications
Suitable for: ELISA, ICC/IF, IP, WBmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Synthetic peptide:
YPYDVPDYA
conjugated to KLH, corresponding to amino acids 98-106 of Influenza A Virus Hemagglutinin HA tag -
Positive control
- Whole extracts of transfected 293T (human embryonal kidney) cells, expressing HA-tagged fusion protein.
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General notes
This product was changed from ascites to tissue culture supernatant on 25th October 2016. The following lot(s) is/are from ascites and is still in stock as of 25th October 2016 - GR275412, GR177296. Lot numbers other than GR275412 and GR177296 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading... -
Purity
Proprietary Purification -
Purification notes
Purified from Tissue culture supernatant”. -
Clonality
Monoclonal -
Clone number
HA-7 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab49969 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA | Use at an assay dependent concentration. Use at an assay dependent concentration. |
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| ICC/IF | Use a concentration of 1 - 2 µg/ml. | |
| IP | Use a concentration of 0.5 - 2 µg/ml. | |
| WB | Use a concentration of 0.25 - 1 µg/ml. Predicted molecular weight: 63 kDa. |
Target
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Relevance
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins. -
Alternative names
- HA epitope tag antibody
- HA1 antibody
- HA2 antibody
see all
Images
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![Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody [HA-7] (ab49969)](https://www.abcam.com/ps/products/49/ab49969/Images/ab49969-273324-anti-ha-tag-antibody-ha-7-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody [HA-7] (ab49969)HEK-293 cells overexpressing N-terminal HA tagged fusion protein were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton™ X-100. Fixed cells were stained with 1 μg/mL Anti-HA tag antibody [HA-7] (ab49969). The antibody was developed using Goat Anti-Mouse IgG, Cy3™ conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
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![Immunoprecipitation - Anti-HA tag antibody [HA-7] (ab49969)](https://www.abcam.com/ps/products/49/ab49969/Images/ab49969-198918-anti-ha-tag-antibody-ha-7-immunoprecipitation.jpg)
Immunoprecipitation - Anti-HA tag antibody [HA-7] (ab49969)This image is courtesy of an anonymous Abreviewab49969 Immunoprecipitating HA tag in HA tagged protein transfected 293T whole cell lysate. 25µg of cell lysate was incubated with primary antibody (1/2500 in lysis buffer) and matrix (Protein A/G) for 6 hours at 4°C. For western blotting anti-mouse HRP (ab6789) (1/10000) was used to confirm successful immunoprecipation.
75kD is specific band for HA tagged protein. 120kD, 55kD, 35kD is non specific band for protein A/G bead.
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![Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody [HA-7] (ab49969)](https://www.abcam.com/ps/products/49/ab49969/Images/ab49969-273325-anti-ha-tag-antibody-ha-7-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody [HA-7] (ab49969)HEK-293 cells overexpressing C-terminal HA tagged fusion protein were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton™ X-100. Fixed cells were stained with 1 μg/mL Anti-HA tag antibody [HA-7] (ab49969). The antibody was developed using Goat Anti-Mouse IgG, Cy3™ conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
Protocols
References
This product has been referenced in:
- Boassa D et al. Split-miniSOG for Spatially Detecting Intracellular Protein-Protein Interactions by Correlated Light and Electron Microscopy. Cell Chem Biol 26:1407-1416.e5 (2019). Read more (PubMed: 31378710) »
- Kim MY et al. Mbd2-CP2c loop drives adult-type globin gene expression and definitive erythropoiesis. Nucleic Acids Res 46:4933-4949 (2018). Read more (PubMed: 29547954) »
