Product nameAnti-HA tag antibody [HA.C5]
See all HA tag primary antibodies
DescriptionMouse monoclonal [HA.C5] to HA tag
Tested applicationsSuitable for: ChIP/Chip, WB, ICC, IP, ICC/IFmore details
This product was changed from ascites to tissue culture supernatant on 5th February 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium Azide
Constituents: PBS, pH 7.4
Concentration information loading...
Purification notesPurified from TCS
ChIP Related Products
Our Abpromise guarantee covers the use of ab18181 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ChIP/Chip||Use at an assay dependent concentration. PubMed: 19581286|
|IP||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.|
RelevanceHuman influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
- HA epitope tag antibody
- HA1 antibody
- HA2 antibody
All lanes : Anti-HA tag antibody [HA.C5] (ab18181) at 1/1000 dilution
Lanes 1-2 : HEK293 whole cell lysate - transfected
Lane 3 : HEK293 whole cell lysate - untransfected
Lysates/proteins at 30 µg per lane.
All lanes : IRDye® 800CW Goat anti-mouse IgG polyclonal at 1/10000 dilution
Performed under reducing conditions.
Observed band size: 85 kDa why is the actual band size different from the predicted?
Exposure time: 5 minutes
ab18181 staining HA tag (green) in HeLa cells by Immunocytochemistry/ Immunofluorescence.
Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton X-100 and blocked with 3% BSA for 1 hour at 22°C. Samples were incubated with primary antibody (1/1000 in diluent) for 1 hour at 22°C. A FITC-conjugated goat anti-mouse polyclonal IgG (1/1000) was used as the secondary antibody. Nuclei were stained with DAPI (blue).
Western blot using ab18181 of 293 cells transfected with HA-tagged vector(2) and untransfected control (1). Western blot using ab18181 of 293 cells transfected with HA-tagged vector(2) and untransfected control (1).
Immunofluorescence using ab18181 staining a HA-tag fusion protein (transcription factor) in a stable expressing cell line (right hand panel) and control cell line (left hand panel).
All lanes : Anti-HA tag antibody [HA.C5] (ab18181) at 1/2000 dilution
Lane 1 : WCE from cell line transfected for HA-tagged protein
Lane 2 : WCE from a cell line transfected with empty vector
Lysates/proteins at 50 µg per lane.
All lanes : HRP conjugated Goat anti-mouse IgG (H+L)
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 10 seconds
Incubation with the primary antibody was carried out at 4°C overnight, whilst the secondary antibody was incubated for 1 hour at room temperature.
This product has been referenced in:
- Cawez F et al. Combinatorial Design of a Nanobody that Specifically Targets Structured RNAs. J Mol Biol 430:1652-1670 (2018). Read more (PubMed: 29654796) »
- Duan Z et al. Importin a5 negatively regulates importin ß1-mediated nuclear import of Newcastle disease virus matrix protein and viral replication and pathogenicity in chicken fibroblasts. Virulence 9:783-803 (2018). Read more (PubMed: 29532715) »