Immunocytochemistry/ Immunofluorescence abreview for Anti-HA tag antibody [HA.C5]

Good
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5% tritonx100
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C

Other product details

Dilution
1/1000
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C

Secondary antibody

Name
Non-Abcam antibody was used: anti-mouse
Host species: Goat
Clonality: Polyclonal
Conjugation: Fluorescein
Dilution
1/1000

Additional data

Additional Notes
HeLa cells were transfected with a HA-Tag protein, and 72 hour post transfection were fixed, permeablized, blocked and stained for HA Tag using ab18181. Gaot anti-mouse FITC tagged antibody was used as secondary antibody

Dr. Harendra Chahar

Verified customer

Submitted Sep 10 2012

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