Western blot abreview for Anti-HA tag antibody [HA.C5]

Excellent
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (293T)
Loading amount
15 µg
Specification
293T
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 8% · Temperature: 20°C

Other product details

Dilution
1/5000
Incubation time
15 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST

Secondary antibody

Name
Non-Abcam antibody was used: HRP-conjugated anti-mouse secondary antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/3000

Detection

Detection method
ECL
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 63 kDa
Positive control
-
Negative control
empty vector

Additional data

Additional Notes
I performed transient transfection with HA-tagging protein.

Mr. Dongil Kim

Verified customer

Submitted Jan 24 2013

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