Immunocytochemistry/ Immunofluorescence abreview for Anti-HA tag antibody [HA.C5]

Excellent
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HepG2)
Specification
HepG2
Fixative
Paraformaldehyde
Permeabilization
Yes - Saponin 0.1%
Blocking step
(agent) for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 19°C

Other product details

Dilution
1/500
Incubation time
1 hour(s) and 0 minute(s) · Diluent: PBS+10% FBS+Saponin 0.1%

Secondary antibody

Name
Non-Abcam antibody was used: Jackson 715-545-150
Host species: Donkey
Clonality: Monoclonal
Conjugation: Alexa Fluor® 488
Dilution
1/400

Additional data

Additional Notes
TFR2 HA tagged protein (c-Term)

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Submitted Feb 12 2013

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