Immunoprecipitation abreview for Anti-HA tag antibody [HA.C5]

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Abreviews
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (CHO cells)
Total protein in input
500 µg
Specification
CHO cells
Immuno-precipitation step
Protein A/G

Other product details

Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C

Western Blot antibody

Name
Non-Abcam antibody was used: HRP-mouse
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000

Additional data

Additional Notes
Cells were infected by retrovirus to produce protein X and western blotting was performed with anti-HA antibody after IP
55kD, 72kD is non-specific band.
100kD specific band for protein X

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Submitted Feb 26 2013

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