Western blot abreview for Anti-HA tag antibody [HA.C5]

Excellent
Abreviews
Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (10)
Sample
Human Cell lysate - whole cell (HEK293)
Specification
HEK293
Blocking step
Odyssey block as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 20°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Odyssey block
Dilution
1/1000

Secondary antibody

Name
Non-Abcam antibody was used: Goat anti-mouse
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye­ 800CW
Dilution
1/10000

Detection

Detection method
LiCor
Bands
Specific: 85 kDa
Exposure
5 minute(s) and 0 second(s)
Negative control
Lane 3 Untransfected cell lysate
Positive control
Lane 1,2 Transfected cell lysate

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Submitted Nov 25 2013

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