Western blot abreview for Anti-HA tag antibody [HA.C5]

Excellent
Abreviews
Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (15%)
Sample
Human Cell lysate - whole cell (Renal cancer cell line)
Specification
Renal cancer cell line
Treatment
Doxicycline induction of Protein_HA tag
Blocking step
Li-cor Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time
2 hour(s) and 0 minute(s) · Temperature: 25°C
Dilution
1/1000

Secondary antibody

Dilution
1/5000

Detection

Detection method
LICOR
Bands
Specific: 25 kDa
Exposure
30 second(s)
Negative control
Non treated cells. Line 1
Positive control
Doxicycline treated cells. Line 2

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Submitted Jul 28 2014

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