Western blot abreview for Anti-HA tag antibody [HA.C5]

Excellent
Abreviews
Application
Western blot
Loading amount
1 µg
Gel Running Conditions
Reduced Denaturing (4-12% gradient)
Sample
Aedes aegypti (Mosquito) Recombinant protein (aedes midgut protein made in E.coli)
Specification
aedes midgut protein made in E.coli
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 5% milk in TBST
Dilution
1/750

Secondary antibody

Name
Non-Abcam antibody was used: Anti-mouse HRP
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000

Detection

Detection method
ECL and film
Bands
Specific: 45 kDa
Exposure
20 second(s)
Positive control
Lane 1 is elution 1, lane 2 is elution 2 - both purified HA-tagged mosquito proteins

Additional data

Additional Notes
Secondary antibody was donkey anti-mouse

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Submitted Mar 31 2015

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