Western blot abreview for Anti-HA tag antibody [HA.C5]

Excellent
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (293T)
Gel Running Conditions
Non-reduced Denaturing (10% SDS)
Loading amount
80 µg
Specification
293T
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C

Other product details

Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk
Dilution
1/1000

Secondary antibody

Dilution
1/2000
Name
Non-Abcam antibody was used: fluorescence conjugated secondary antibody
Host species: Goat
Clonality: Monoclonal
Conjugation: Fluorescein

Detection

Detection method
Odyssey
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 42-95 kDa

Qingyuan Yang

Verified customer

Submitted Dec 30 2015

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