Western blot abreview for Anti-HA tag antibody [HA.C5]

Excellent
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (Renal Carcinoma Cell Line)
Loading amount
50 µg
Specification
Renal Carcinoma Cell Line
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4%

Other product details

Dilution
1/2000
Incubation time
16 hour(s) and 0 minute(s)

Secondary antibody

Name
Non-Abcam antibody was used: Pierce Goat anti-mouse IgG(H+L)
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL+
Exposure
10 second(s)
Bands
Specific: 28 kDa
Positive control
WCE from cell line transfected for HA-tagged protein
Negative control
WCE from a cell line transfected with empty vector

Additional data

Additional Notes
10% reducing gel, transferred to PVDF membrane, incubated primary overnight rocking at 4C, blocked with 4% skim milk, 5X 5min wash with TBS-T, HRP secondary incubated 1 hour at room temperature, 5X 5min wash with TBS-T, visualized with ECL+

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Submitted Jul 26 2006

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